{
  "abstract": "Myelinating oligodendrocytes are first observed during evolution in vertebrates above jawless fishes. Oligodendrocyte progenitor cells (OPCs) undergo extensive transcriptional and morphological remodelling to initiate differentiation and myelination. We report that the zinc-finger transcription factor St18 (aka Myt3) is upregulated during the OPC-to–newly formed oligodendrocyte (NFOL) transition. Single-nucleus RNA-sequencing (snRNA-seq) performed after lysolecithin-induced demyelination of the mouse spinal cord revealed that St18 was acutely upregulated in remyelinating OPCs. In primary rat OPC cultures, St18 overexpression promoted differentiation, whereas OPC conditional St18 knockout delayed developmental myelination in the mouse brain. Chromatin accessibility profiling identified candidate cis-regulatory elements for oligodendroglial St18 expression activated by core oligodendrocyte transcription factors, including SOX10, KLF9/13, and MYRF, and we confirmed that St18 expression in OPCs is Sox10-dependent in vivo. Phylogenetic analysis suggests that St18 arose via duplication of Myt1 in early jawed vertebrates, coinciding with the emergence of myelinating oligodendrocytes. These findings define St18 as a transcriptional marker of NFOLs and OLs and provide insight into the evolutionary regulation of central nervous system myelination.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 21,
  "authors": [
    {
      "name": "David Rowitch",
      "orcid": "0000-0002-0079-0060"
    },
    {
      "name": "Daniel Yamamoto"
    },
    {
      "name": "Adrien Vaquié"
    },
    {
      "name": "Tim Aberle",
      "orcid": "0000-0002-5872-6272"
    },
    {
      "name": "Michael Perry"
    },
    {
      "name": "Davod Shah"
    },
    {
      "name": "Diana Gold Diaz"
    },
    {
      "name": "Guy Lam"
    },
    {
      "name": "Zhaoyang Xu"
    },
    {
      "name": "Gregory Jordan"
    },
    {
      "name": "Gemma Girdler"
    },
    {
      "name": "Hannah Wüst"
    },
    {
      "name": "Laura Morcom"
    },
    {
      "name": "Aimee Wilson"
    },
    {
      "name": "Khalil Rawji"
    },
    {
      "name": "Penelope Dimas"
    },
    {
      "name": "Guoqiang Gu",
      "orcid": "0000-0003-0772-9139"
    },
    {
      "name": "Sarah Teichmann",
      "orcid": "0000-0002-6294-6366"
    },
    {
      "name": "Robin Franklin",
      "orcid": "0000-0001-6522-2104"
    },
    {
      "name": "Ragnhildur Káradóttir",
      "orcid": "0000-0001-9675-2722"
    },
    {
      "name": "Michael Wegner"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/80wsx3k442mfw.bib",
  "cited_by": 0,
  "doi": "10.21203/rs.3.rs-11211282/v1",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13734250/",
  "id": "80wsx3k442mfw",
  "issns": [
    "2693-5015"
  ],
  "landing_url": "https://doi.org/10.21203/rs.3.rs-11211282/v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2806|Neurogenesis and neuroplasticity mechanisms"
    ]
  ],
  "openalex": "W7224078949",
  "pdf_url": null,
  "pmcid": "PMC13734250",
  "pmid": "42990742",
  "published": "2026-10-29",
  "publisher": "American Journal Experts",
  "ris": "https://research.draconitic.com/paper/80wsx3k442mfw.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:23:52Z",
      "native_id": "PMC13734250",
      "source": "pmc",
      "url": "https://doi.org/10.21203/rs.3.rs-11211282/v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2806|Neurogenesis and neuroplasticity mechanisms",
      "subfield": "neuroscience"
    }
  ],
  "title": "Regulation, function and evolutionary structure of St18, encoding a Zinc-Finger Transcription Factor, in the oligodendrocyte lineage",
  "title_text": "Regulation, function and evolutionary structure of St18, encoding a Zinc-Finger Transcription Factor, in the oligodendrocyte lineage",
  "type": "preprint",
  "updated_at": "2026-11-08T12:23:52Z",
  "url": "https://research.draconitic.com/paper/80wsx3k442mfw",
  "venue": "Research Square",
  "versions": []
}