{
  "abstract": "Oxidized graphene quantum dots (o-GQDs) alter viability and cell-cycle progression in breast cancer cells, yet the cellular programs underlying these effects remain incompletely defined. We exposed MCF-7 cells to 10 mg/L o-GQD for 72 h and integrated RNA sequencing with quantitative protein-marker, ROS, antioxidant-intervention, proteasome-inhibition, and puromycin-labeling analyses from independent experiments. Transcriptomic analyses showed significant negative enrichment of proliferative and cell-cycle programs (E2F Targets, G2M Checkpoint, MYC Targets V1), with complementary KEGG/Reactome evidence of attenuated estrogen-associated programs. Quantitative Western blotting showed dose-dependent reductions in ERα (to 41.36 ± 13.73% at 10 mg/L), p-RB (to 8.31 ± 10.39%), and Survivin (to 23.97 ± 19.01%), with p-ERK/ERK attenuation and marked γ-H2AX accumulation (345.21 ± 161.39%). The ROS-positive fraction rose from 6.86% to 30.46%; antioxidant co-treatment failed to rescue the phenotype and MG132 did not consistently restore protein markers, arguing against oxidative stress or proteasomal degradation as standalone drivers. Reduced puromycin labeling added a protein-homeostasis readout consistent with reduced nascent protein synthesis. BT-474 and MDA-MB-231 cells showed weaker or different marker responses. Together, these findings define a coordinated MCF-7 response spanning estrogen-associated and cell-cycle programs, survival markers, protein homeostasis, and cell fate.",
  "abstract_shown": true,
  "affiliations": [
    "Laboratory of Molecular and Surgical Research, Department of Research, Changhua Christian Hospital, Changhua 50050, Taiwan",
    "Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wei-Chung Shia",
      "orcid": "0000-0001-5351-840X"
    },
    {
      "affs": [
        1
      ],
      "name": "Chien-Te Hsieh",
      "orcid": "0000-0002-1053-8635"
    },
    {
      "affs": [
        1
      ],
      "name": "Po-Chih Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Wan-Ling Chuang",
      "orcid": "0000-0001-8815-3834"
    },
    {
      "affs": [
        0
      ],
      "name": "Wan-Ting Shen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/81hg4zs9xb2hv.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27209211",
  "fulltext_url": "https://europepmc.org/articles/PMC13705060",
  "id": "81hg4zs9xb2hv",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/9211",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Graphene and Nanomaterials Applications"
    ]
  ],
  "openalex": "W7222760028",
  "pdf_url": "https://europepmc.org/articles/PMC13705060?pdf=render",
  "pmcid": "PMC13705060",
  "pmid": "42941980",
  "published": "2026-10-16",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/81hg4zs9xb2hv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:57Z",
      "native_id": "10.3390/ijms27209211",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/9211"
    },
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42941980",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42941980"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "Integrated Transcriptomic and Biochemical Analyses Reveal Coordinated Suppression of ER-Associated Survival Signaling and Activation of ROS-Mediated Stress Responses by Oxidized Graphene Quantum Dots in Estrogen Receptor-Positive Breast Cancer",
  "title_text": "Integrated Transcriptomic and Biochemical Analyses Reveal Coordinated Suppression of ER-Associated Survival Signaling and Activation of ROS-Mediated Stress Responses by Oxidized Graphene Quantum Dots in Estrogen Receptor-Positive Breast Cancer",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/81hg4zs9xb2hv",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}