{
  "abstract": "Here we describe an automated workflow using a PAL Robotic Tool Changer (RTC) system for plasma lipid sample preparation and off-line two-dimensional liquid chromatography (LCxLC) separation. The orthogonal chromatographic platform couples hydrophilic interaction liquid chromatography (HILIC) in the first dimension with rapid reversed-phase liquid chromatography (RPLC) in the second dimension, facilitated by automated fraction collection at 10-second intervals into 96-well plates. Lipid profiling and structural identification strategies were evaluated using collision-induced dissociation (CID) and electron-activated dissociation (EAD) across data-dependent acquisition (DDA), dataindependent acquisition (DIA / SWATH), and high-resolution multiple reaction monitoring (HRMRM) modes. Standard Reference Material NIST SRM 1950 (Human Plasma) was employed for lipid quantification and double-bond characterization of phosphatidylcholines (PCs). By combining automated modified Matyas liquid-liquid extraction, and off-line LCxLC, a total of 115 PC’s lipids could be detected in SRM 1950 plasma sample. For 74 PC’s including isomers, double assignments could be proposed based on the EAD spectra.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Inorganic and Analytical Chemistry",
    "University of Geneva",
    "CTC Analytics AG"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Laura Gisela González Iglesias"
    },
    {
      "affs": [
        2
      ],
      "name": "Renzo Picenoni"
    },
    {
      "affs": [
        2
      ],
      "name": "Guenter Boehm"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Gérard Hopfgartner"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/81sycvh2d38g6.bib",
  "cited_by": 0,
  "doi": "10.26434/chemrxiv.15009982/v1",
  "fulltext_url": null,
  "id": "81sycvh2d38g6",
  "issns": [],
  "landing_url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009982/v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Metabolomics and Mass Spectrometry Studies"
    ]
  ],
  "openalex": "W7220737595",
  "pdf_url": "https://chemrxiv.org/doi/pdf/10.26434/chemrxiv.15009982/v1",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-06",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/81sycvh2d38g6.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:22Z",
      "native_id": "10.26434/chemrxiv.15009982",
      "source": "chemrxiv",
      "url": "https://chemrxiv.org/doi/full/10.26434/chemrxiv.15009982/v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Metabolomics and Mass Spectrometry Studies",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Off-line LCxLC-Mass Spectrometry Workflow using CID and EAD for the Quantification and Comprehensive Structural Double Bond Characterization of Glycerophospholipids in Plasma Samples",
  "title_text": "Off-line LCxLC-Mass Spectrometry Workflow using CID and EAD for the Quantification and Comprehensive Structural Double Bond Characterization of Glycerophospholipids in Plasma Samples",
  "type": "preprint",
  "updated_at": "2026-10-30T12:17:22Z",
  "url": "https://research.draconitic.com/paper/81sycvh2d38g6",
  "venue": "ChemRxiv",
  "versions": []
}