{
  "abstract": "The eicosanoid PGE₂ upregulates intracellular Ca²⁺/cAMP levels and induces a cellular immune response, whereas EpOME inhibits PGE₂ activity in insects. However, the molecular interactions between the two antagonistic ligands remain largely unexplored. Here we identify nine homologs of vertebrate C18-oxylipin-associating GPCRs among the GPCRs of Spodoptera exigua (SeGPCRs). Functional homology is validated through RNA interference screening, followed by heterologous expressions in both directions between insect and human cell lines. A deletion mutant of SeGPCR48, generated via CRISPR/Cas9, loses responsiveness to EpOME, which is confirmed functionally and by differential gene expression analysis following RNA-seq. In silico binding assay indicate high binding affinity of EpOME to SeGPCR48, which is supported by experimental follow-up, involving ligand-binding functional assays and point mutagenesis of two residues in the binding pocket. In congruence with the in vitro experiments, the EpOME-SeGPCR48 ligand-receptor pair showed marked inhibitory activity against PGE₂-mediated immunity. Collectively, this study identifies an insect EpOME receptor within an orphan GPCR subfamily that is phylogenetically conserved across metazoans.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life Science & Engineering, Gyeongkuk National University, Andong, Korea.",
    "Division of Biomedical Metrology, Korea Research Institute of Standards and Science, Daejeon, Korea.",
    "Industry Academy Cooperation Foundation, Gyeongkuk National University, Andong, Korea.",
    "Department of Pharmacy, Section for Pharmaceutical Chemistry, University of Oslo, Oslo, Norway.",
    "School of Life Science & Engineering, Gyeongkuk National University, Andong, Korea. hosanna@gknu.ac.kr."
  ],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Niayesh Shahmohammadi",
      "orcid": "0000-0003-1875-1227"
    },
    {
      "affs": [
        0
      ],
      "name": "Falguni Khan"
    },
    {
      "affs": [
        1
      ],
      "name": "Haneol Jang"
    },
    {
      "affs": [
        1
      ],
      "name": "Il-Hwan Kim"
    },
    {
      "affs": [
        2
      ],
      "name": "Donghee Lee"
    },
    {
      "affs": [
        3
      ],
      "name": "Joseph Wallen"
    },
    {
      "affs": [
        0
      ],
      "name": "Hyeok Jin Choi"
    },
    {
      "affs": [
        0
      ],
      "name": "Jin Boo Jeong",
      "orcid": "0000-0002-0936-341X"
    },
    {
      "affs": [
        3
      ],
      "name": "Anders Vik",
      "orcid": "0000-0002-7864-3271"
    },
    {
      "affs": [
        4
      ],
      "name": "Yonggyun Kim",
      "orcid": "0000-0002-6840-2167"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/82ndv2m98c25p.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-77858-6",
  "fulltext_url": "https://europepmc.org/articles/PMC13687035",
  "id": "82ndv2m98c25p",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://europepmc.org/article/MED/42907432",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2804|Neurobiology and Insect Physiology Research"
    ]
  ],
  "openalex": "W7214022689",
  "pdf_url": "https://europepmc.org/articles/PMC13687035?pdf=render",
  "pmcid": "PMC13687035",
  "pmid": "42907432",
  "published": "2026-09-22",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/82ndv2m98c25p.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:57Z",
      "native_id": "MED:42907432",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42907432"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2804|Neurobiology and Insect Physiology Research",
      "subfield": "neuroscience"
    }
  ],
  "title": "EpOME functionally antagonises prostaglandin signalling in Spodoptera exigua via a specific GPCR conserved across metazoans.",
  "title_text": "EpOME functionally antagonises prostaglandin signalling in Spodoptera exigua via a specific GPCR conserved across metazoans.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:57Z",
  "url": "https://research.draconitic.com/paper/82ndv2m98c25p",
  "venue": "Nature communications",
  "versions": []
}