{
  "abstract": "DNA-stabilized silver nanoclusters (DNA-AgNCs) combine molecular-scale dimensions with sequence-dependent optical properties, making them promising candidates for bioimaging applications. However, strategies for site-specific conjugation of DNA-AgNCs remain comparatively limited. Here, we expand the conjugation toolbox of DNA-AgNCs through biotin functionalization of previously developed single-stranded DNA-AgNC containing a [Ag16Cl2]8+ cluster. Incorporation of biotin either at 3′ end or in the middle of the sequence, enabled selective immobilization of the resulting biotinylated DNA-AgNCs on streptavidin-coated poly(methyl methacrylate) (PMMA) microparticles. Importantly, biotin functionalization and subsequent particle attachment largely preserved the characteristic optical properties and fluorescence lifetimes of the DNA-AgNCs, while control experiments showed negligible nonspecific binding of the non-biotinylated DNA-AgNCs. These results demonstrated that biotin–streptavidin interaction provides a robust strategy for the modular functionalization of DNA-AgNCs without compromising their photophysical properties, opening opportunities for their integration into future bioimaging and sensing applications.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemistry, University of Copenhagen Universitetsparken 5 2100 Copenhagen Denmark",
    "Department of Forensic Medicine, University of Copenhagen Frederik V's Vej 11 2100 Copenhagen Denmark"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Vanessa Rück",
      "orcid": "0000-0002-7403-3118"
    },
    {
      "affs": [
        0
      ],
      "name": "Simon Wenzel Lind",
      "orcid": "0009-0005-6207-3621"
    },
    {
      "affs": [
        1
      ],
      "name": "Christian Brinch Mollerup",
      "orcid": "0000-0001-8187-6474"
    },
    {
      "affs": [
        0
      ],
      "name": "Tom Vosch",
      "orcid": "0000-0001-5435-2181"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/82t1axgdmftgm.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra09400g",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13722049/?tool=EBI",
  "id": "82t1axgdmftgm",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13722049",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemistry"
    ],
    [
      "openalex",
      "2505|Nanocluster Synthesis and Applications"
    ]
  ],
  "openalex": "W7224844713",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13722049/pdf/?tool=EBI",
  "pmcid": "PMC13722049",
  "pmid": "42965164",
  "published": "2026-10-30",
  "publisher": "Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/82t1axgdmftgm.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:36Z",
      "native_id": "PMC:PMC13722049",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13722049"
    },
    {
      "first_seen": "2026-11-03T12:22:38Z",
      "native_id": "PMC13722049",
      "source": "pmc",
      "url": "https://doi.org/10.1039/d6ra09400g"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2505|Nanocluster Synthesis and Applications",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Expanding the conjugation toolbox of DNA-stabilized silver nanoclusters with biotin-streptavidin",
  "title_text": "Expanding the conjugation toolbox of DNA-stabilized silver nanoclusters with biotin-streptavidin",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:22:38Z",
  "url": "https://research.draconitic.com/paper/82t1axgdmftgm",
  "venue": "RSC advances",
  "versions": []
}