{
  "abstract": "This study investigated the dose-dependent effects of a lactobacilli-based postbiotic ( Post ) on the transcriptional reprogramming of the chicken macrophage-like HD11 cell line when exposed to Escherichia coli lipopolysaccharide ( LPS ). First, the HD11cells were treated with 0, 3, 30 and 300 ng/mL LPS in combination with 0, 0.2, 0.4, 0.6 and 0.8% v/v Post. Nitric oxide ( NO ) production was quantified at 20 h incubation and the early transcriptome reprogramming was analysed in a subset of treatments at 5 h incubation. Results Post increased NO production dose-dependently and an LPS-postbiotic interaction was present, with the cells eliciting a higher NO production in response to Post at 30 and 300 ng/ml LPS compared to the zero and 3 ng/ml LPS. To further understand this interaction, the early transcriptome reprogramming was investigated for treatments with 0, 3 and 300 ng/mL LPS and 0 and 0.8% v/v Post. A number of differentially expressed genes were identified and gene set enrichment analysis of KEGG pathways revealed that Post at 0 and 300 ng/mL LPS influenced similar inflammation-related pathways, until Post at 3 ng/mL LPS which had a minimal effect. Expression of transcription factors ( TFs ) and immune-related genes revealed differential effects induced by Post depending on LPS concentration which would have likely influenced the inflammatory response. Specifically, the only TFs affected by Post at 300 ng/ml LPS were STAT2 , SMAD3 and IFR8 , which all showed increased expression. The TFs consistently affected by Post at the zero and 3 ng/ml LPS increased and were JUN , ZFP36L2 , SMAD1 and E2F3 . Conclusion Our results showed that Post had a pro-inflammatory effect, which was exacerbated in the presence of a 300 but not 3 ng/ml LPS. Furthermore, the dose of LPS affected the sensitivity of the cells to Post. Dose-response studies should be performed when investigating the effects of dietary compounds on inflammation in chicken macrophages.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "Samuel Jansseune"
    },
    {
      "name": "Jürgen van Baal"
    },
    {
      "name": "Fany Blanc"
    },
    {
      "name": "Aart Lammers"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/84n8s8rv8mtay.bib",
  "cited_by": 1,
  "doi": "10.1186/s12917-025-04902-w",
  "fulltext_url": null,
  "id": "84n8s8rv8mtay",
  "issns": [
    "1746-6148"
  ],
  "landing_url": "https://hal.inrae.fr/hal-05447387v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1106|Probiotics and Fermented Foods"
    ]
  ],
  "openalex": "W4412505713",
  "pdf_url": "https://hal.inrae.fr/hal-05447387/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-29",
  "publisher": "BioMed Central",
  "ris": "https://research.draconitic.com/paper/84n8s8rv8mtay.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:03Z",
      "native_id": "hal-05447387",
      "source": "hal",
      "url": "https://hal.inrae.fr/hal-05447387v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage",
  "title_text": "Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:20:56Z",
  "url": "https://research.draconitic.com/paper/84n8s8rv8mtay",
  "venue": "BMC Veterinary Research",
  "versions": []
}