{
  "abstract": "Introduction: Salinity and drought are major abiotic stresses limiting pea ( Pisum sativum L. ) growth and productivity. The RCI2 family has been implicated in plant responses to environmental stress, but its characteristics and potential roles in pea remain insufficiently understood.\n\nMethods: Six PsRCI2 genes were identified and characterized using phylogenetic, gene structure, promoter, transcriptomic, quantitative real-time PCR (qRT-PCR), and subcellular localization analyses.\n\nResults: PsRCI2-2 to PsRCI2-5 formed a gene cluster on chromosome 3, whereas no clear intragenomic collinear pairs were detected. Most PsRCI2 proteins showed conserved sequence and transmembrane features, whereas PsRCI2-6 exhibited distinct structural characteristics. Promoter analysis revealed diverse stress- and hormone-responsive cis-acting elements among family members. Transcriptome analysis revealed differential transcriptional responses to salt and PEG-induced osmotic stress. Notably, PsRCI2-2 , PsRCI2-3 , and PsRCI2-5 were induced by both treatments, and their expression patterns were validated by qRT-PCR. These three proteins also exhibited fluorescence patterns largely overlapping with the ER marker in Nicotiana benthamiana .\n\nDiscussion: The pea RCI2 family displays conserved structural features but divergent transcriptional responses. PsRCI2-2 , PsRCI2-3 , and PsRCI2-5 are promising candidates for further functional studies investigating pea responses to abiotic stress.",
  "abstract_shown": true,
  "affiliations": [
    "Biotechnology Research Institute, Chongqing Academy of Agricultural Sciences",
    "Tianjin Customs Animal, Plant and Food Inspection Center"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Mingyuan Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yongdong Dai"
    },
    {
      "affs": [
        1
      ],
      "name": "Rui Yang"
    },
    {
      "affs": [
        0
      ],
      "name": "Hongjuan Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/852p0wt17a43y.bib",
  "cited_by": 0,
  "doi": "10.3389/fpls.2026.2003888",
  "fulltext_url": null,
  "id": "852p0wt17a43y",
  "issns": [
    "1664-462X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fpls.2026.2003888/full",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-29",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/852p0wt17a43y.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:25Z",
      "native_id": "10.3389/fpls.2026.2003888",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fpls.2026.2003888/full"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:1664-462X",
      "subfield": "plant-biology"
    }
  ],
  "title": "Genome-wide characterization of the RCI2 gene family reveals conserved protein features and divergent responses to salt and osmotic stress in pea",
  "title_text": "Genome-wide characterization of the RCI2 gene family reveals conserved protein features and divergent responses to salt and osmotic stress in pea",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T00:20:59Z",
  "url": "https://research.draconitic.com/paper/852p0wt17a43y",
  "venue": "Frontiers in Plant Science",
  "versions": []
}