{
  "abstract": "Time-resolved crystallography can be inaccessible for membrane proteins and other difficult-to-crystallize targets for which suitable crystals are scarce. Here we present an acquisition strategy that records consecutive diffraction images during a small crystal rotation synchronized to a single activation pulse. Matching image segments are merged across crystals to reconstruct successive time windows, enabling a single crystal ensemble to capture an extended structural trajectory. Using the Mesh&Collect workflow at a synchrotron, we obtained ten consecutive 7.5-ms time windows at 2 [A] resolution from 1,255 microcrystals of a bacteriorhodopsin triple mutant at room temperature. An additional 790 crystals extended the observation window to 500 ms. Rotation-based processing, retrospective time binning and dose control maximize information extracted from each crystal, while compatible measurements can be pooled across acquisitions. This approach makes millisecond time-resolved crystallography practical for crystal-limited targets at synchrotrons, with further development potentially extending the temporal resolution down to 1 ms.",
  "abstract_shown": true,
  "affiliations": [
    "European Synchrotron Radiation Facility"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "S. Bukhdruker"
    },
    {
      "name": "R. Astashkin"
    },
    {
      "name": "D. Bratanov"
    },
    {
      "name": "N. Caramello"
    },
    {
      "name": "S. Engilberge"
    },
    {
      "name": "C. Baeken"
    },
    {
      "name": "T. Balandin"
    },
    {
      "name": "A. Popov"
    },
    {
      "name": "A. Royant"
    },
    {
      "name": "C. Mueller-Dieckmann"
    },
    {
      "name": "M. Soler-Lopez"
    },
    {
      "name": "E. Bamberg"
    },
    {
      "name": "V. Gordeliy"
    },
    {
      "name": "I. Melnikov"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/858kw2g56jsaa.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.04.756486",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.04.756486v1.full",
  "id": "858kw2g56jsaa",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.04.756486v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biophysics"
    ],
    [
      "openalex",
      "2505|Enzyme Structure and Function"
    ]
  ],
  "openalex": "W7221119977",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.04.756486v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-04",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/858kw2g56jsaa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:47Z",
      "native_id": "10.64898/2026.10.04.756486",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.04.756486v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biophysics",
      "subfield": "biophysics"
    }
  ],
  "title": "Sample-efficient time-resolved X-ray crystallography with serial small wedges",
  "title_text": "Sample-efficient time-resolved X-ray crystallography with serial small wedges",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:47Z",
  "url": "https://research.draconitic.com/paper/858kw2g56jsaa",
  "venue": "bioRxiv",
  "versions": []
}