{
  "abstract": "Standard methods for measuring red blood cell (RBC) deformability, such as ektacytometry, are rarely available in routine clinical practice. We analyzed near wall impedance pulses generated by RBCs in a Coulter system without hydrodynamic focusing, designed for Complete Blood Count (CBC). The relative amplitude of the rotation associated peak (F) was computed, and its interquartile range (ΔQ_F) was proposed as a global deformability marker. Comparisons with ektacytometry were performed under controlled modulation of RBC deformability (glutaraldehyde, NS309) and in patient samples (15 transfused patients with sickle cell disease (SCD), 26 non-transfused SCD patients (22 HbSS and 4 HbSC), 20 diabetes patients, 7 individuals with secondary polycythemia, 4 with hereditary spherocytosis and 24 healthy subjects). A digital twin of the Coulter counter was used to support the analysis. ΔQ_F increased dose-dependently with glutaraldehyde and NS309 treatment and the values correlated with RBC deformability determined by ektacytometry (|r|>0.95, p<0.001). ΔQ_F was significantly higher in HbSS and HbSC patients (p<0.001), and to a lesser degree in transfused SCD patients (p<0.05), compared to controls. No significant differences were observed between individuals with diabetes or polycythemia and controls. ΔQ_F strongly correlated with several parameters reflecting RBC deformability and determined by ektacytometry, the gold standard technique (|r|> 0.79, p < 0.001), supporting its relevance as a rigidity marker. ΔQ_F provides RBC deformability information during routine CBC and may support the development of a practical “rigidity flag” for SCD screening and monitoring in compatible hematology analyzers. This approach could help integrate RBC rheology into routine hematology workflows.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Pierre Taraconat"
    },
    {
      "name": "Maëva Rage"
    },
    {
      "name": "Pierre Pottier"
    },
    {
      "name": "Elie Nader"
    },
    {
      "name": "Damien Isebe"
    },
    {
      "name": "Jean-Philippe Gineys"
    },
    {
      "name": "Nevzat Temurok"
    },
    {
      "name": "Franck Nicoud"
    },
    {
      "name": "Philippe Connes"
    },
    {
      "name": "Simon Mendez"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/86aty34jwvqjq.bib",
  "cited_by": 0,
  "doi": null,
  "fulltext_url": null,
  "id": "86aty34jwvqjq",
  "issns": [
    "3050-5984",
    "3050-5984"
  ],
  "landing_url": "https://hal.science/hal-05801488v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.phys"
    ],
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.phys.meca"
    ],
    [
      "hal",
      "1.phys.phys"
    ],
    [
      "hal",
      "1.sdv.mhep"
    ],
    [
      "hal",
      "2.phys.meca.biom"
    ],
    [
      "hal",
      "2.phys.phys.phys-bio-ph"
    ],
    [
      "hal",
      "2.sdv.mhep.hem"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://hal.science/hal-05801488/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-15",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/86aty34jwvqjq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:57Z",
      "native_id": "hal-05801488",
      "source": "hal",
      "url": "https://hal.science/hal-05801488v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    },
    {
      "field": "medicine",
      "origin": "hal:1.sdv.mhep",
      "subfield": "general"
    },
    {
      "field": "physics",
      "origin": "hal:2.phys.phys.phys-bio-ph",
      "subfield": "biological-medical"
    }
  ],
  "title": "Impedance Signals Reveal Red Blood Cell Deformability: Comparison with Ektacytometry",
  "title_text": "Impedance Signals Reveal Red Blood Cell Deformability: Comparison with Ektacytometry",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:57Z",
  "url": "https://research.draconitic.com/paper/86aty34jwvqjq",
  "venue": "Blood Red Cells & Iron",
  "versions": []
}