{
  "abstract": "Plant cell wall polysaccharide-degrading enzymes (PCWPEs) used in industry are predominantly of microbial origin. Key enzyme classes, including cellulases, lytic polysaccharide monooxygenases (LPMOs), β-xylanases, β-mannanases and pectinases, have attained technological maturity and are widely deployed across agro-waste biorefineries, food and feed processing, textile and pulp manufacturing. Low catalytic efficiency, insufficient thermostability, poor operational adaptability, restricted functionality, and product inhibition limit the industrial use of these enzymes. Structure- and sequence-guided design, supported by computational design tools, now allows more targeted improvement of PCWPE performance. Yet, the stringent and context-specific performance requirements imposed by diverse industrial applications continue to present substantial challenges. This review systematically summarizes current engineering strategies for PCWPEs in the past decade, aiming to provide a comprehensive understanding of the tailored strategies employed in different engineering directions and to offer insightful proposals for future research.",
  "abstract_shown": true,
  "affiliations": [
    "China Tobacco Jiangsu Industrial Co., Ltd.",
    "State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry, Nanjing University",
    "Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University"
  ],
  "article_type": "review article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Zihao Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Liangtao Xu"
    },
    {
      "affs": [
        1
      ],
      "name": "Jun Zhou"
    },
    {
      "affs": [
        2
      ],
      "name": "Renjun Gao"
    },
    {
      "affs": [
        0
      ],
      "name": "Huaibin Shi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/87y3nzh4dgtd0.bib",
  "cited_by": 0,
  "doi": "10.3389/fmicb.2026.1945309",
  "fulltext_url": "https://europepmc.org/articles/PMC13710976",
  "id": "87y3nzh4dgtd0",
  "issns": [
    "1664-302X"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1945309/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Review"
    ],
    [
      "openalex",
      "1305|Enzyme Production and Characterization"
    ]
  ],
  "openalex": "W7221618959",
  "pdf_url": "https://europepmc.org/articles/PMC13710976?pdf=render",
  "pmcid": "PMC13710976",
  "pmid": "42948011",
  "published": "2026-10-13",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/87y3nzh4dgtd0.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:12Z",
      "native_id": "10.3389/fmicb.2026.1945309",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fmicb.2026.1945309/full"
    },
    {
      "first_seen": "2026-10-31T00:20:34Z",
      "native_id": "MED:42948011",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42948011"
    },
    {
      "first_seen": "2026-10-31T12:21:50Z",
      "native_id": "PMC13710976",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fmicb.2026.1945309"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1305|Enzyme Production and Characterization",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Unleashing the industrial potential of microbial plant cell wall polysaccharide-degrading enzymes: recent advances in protein engineering",
  "title_text": "Unleashing the industrial potential of microbial plant cell wall polysaccharide-degrading enzymes: recent advances in protein engineering",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:50Z",
  "url": "https://research.draconitic.com/paper/87y3nzh4dgtd0",
  "venue": "Frontiers in Microbiology",
  "versions": []
}