{
  "abstract": "Bone metastasis of lung adenocarcinoma (LUAD) directly leads to poor prognosis. This study aimed to dissect the stromal landscape of LUAD with bone metastases. Unsupervised clustering of scRNA-seq data from LUAD patients with bone metastasis identifies stromal subsets. Pseudotime analysis combined with CytoTRACE stemness scoring reconstructs mesenchymal stromal cell (MSC) differentiation trajectories, while CellChat characterizes cell-cell communication networks among MSC subpopulations. Differentially expressed genes (DEGs) specific to functionally distinct MSC subsets are subjected to LASSO-Cox regression for construction of a prognostic RiskScore model, which is validated in independent bulk RNA-seq cohorts regarding overall survival, immune cell infiltration, and drug sensitivity. A distinct high-stemness MSC C1 subpopulation is localized at the bifurcation point of two divergent differentiation trajectories toward a matrix-remodeling fate and a secretory, immunomodulatory phenotype. The RiskScore model stratifies patients into low- and high-risk groups with divergent overall-survival outcomes. Reduced infiltration of cytotoxic lymphocytes is observed in high-risk patients, and higher drug sensitivity within the high-risk subgroup. In vitro assays verify that LDHA knockdown impairs proliferation and migration in A549 cells. This study provides a rationale for targeting stroma-derived genes in risk-adapted therapy.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Orthopedics, Shanghai Geriatric Medical Center, Shanghai, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Houlei Wang",
      "orcid": "0009-0003-9134-7244"
    },
    {
      "affs": [
        0
      ],
      "name": "Tianle Ma"
    },
    {
      "affs": [
        0
      ],
      "name": "Chao Jia",
      "orcid": "0009-0008-9726-493X"
    },
    {
      "affs": [
        0
      ],
      "name": "Chunhui Ma"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/885qh7z4hcdnq.bib",
  "cited_by": 0,
  "doi": "10.1111/cbdd.70414",
  "fulltext_url": "https://europepmc.org/articles/PMC13680633",
  "id": "885qh7z4hcdnq",
  "issns": [
    "1747-0277",
    "1747-0285"
  ],
  "landing_url": "https://europepmc.org/article/MED/42897747",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2730|Cancer Cells and Metastasis"
    ]
  ],
  "openalex": "W7223037717",
  "pdf_url": "https://europepmc.org/articles/PMC13680633?pdf=render",
  "pmcid": "PMC13680633",
  "pmid": "42897747",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/885qh7z4hcdnq.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:37Z",
      "native_id": "MED:42897747",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42897747"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2730|Cancer Cells and Metastasis",
      "subfield": "oncology"
    }
  ],
  "title": "MSC C1: A Potential Hub Linking Stromal Plasticity, Immune Regulation, and Patient Survival in Lung Adenocarcinoma.",
  "title_text": "MSC C1: A Potential Hub Linking Stromal Plasticity, Immune Regulation, and Patient Survival in Lung Adenocarcinoma.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:37Z",
  "url": "https://research.draconitic.com/paper/885qh7z4hcdnq",
  "venue": "Chemical biology & drug design",
  "versions": []
}