{
  "abstract": "Inter‐organ communication is governed by a complex “secretome,” yet mapping the journey of these factors from their origin to precise cellular destinations remains a fundamental challenge. Proximity labeling emerges as a powerful tool to dissect the secretome, yet conventional workflows typically rely on single‐compartment biotinylation at the endoplasmic reticulum (ER), failing to capture proteins that utilize unconventional secretion. Here, we present DuO‐SCOUT (Dual‐Organelle Secretome Conjugation and Organ‐Uptake Tracking), a high‐performance platform that simultaneously targets BioID2 to the ER and the trans‐Golgi network (TGN). This integrated strategy captures the full secretory maturation relay, increasing protein identification by over 120% compared to traditional ER‐anchored methods. We translated this in vivo using an Adipoq‐Cre mouse model to map the adipose secretome. To bridge the gap between systemic transport and tissue‐specific uptake, DuO‐SCOUT integrates BSPA (Biotin‐Specific Proximity Amplification), a visualization toolkit detecting biotinylated proteins with sub‐nanomolar sensitivity. In obese mice, we identified the piriform cortex (PIR) as a previously unrecognized extra‐hypothalamic sink for adipose‐derived leptin. This is associated with a localized neuroinflammatory signature, including significant induction of Il6. DuO‐SCOUT establishes a broadly applicable framework for dissecting the complex molecular logic of systemic organ‐organ communication.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biomedical Sciences College of Biomedicine City University of Hong Kong Hong Kong Hong Kong SAR China",
    "Biotech and Health Centre Shenzhen Research Institute of City University of Hong Kong Shenzhen Guangdong China",
    "Department of Neuroscience College of Biomedicine City University of Hong Kong Hong Kong Hong Kong SAR China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Fenglian Yang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Rui Qian"
    },
    {
      "affs": [
        2
      ],
      "name": "Jiaxing Song"
    },
    {
      "affs": [
        0
      ],
      "name": "Xingqi Meng"
    },
    {
      "affs": [
        0
      ],
      "name": "Jin Young Kim",
      "orcid": "0000-0003-4438-1872"
    },
    {
      "affs": [
        2
      ],
      "name": "Kwok On Lai",
      "orcid": "0000-0002-4069-054X"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Li Wang",
      "orcid": "0000-0002-3377-8092"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Liang Zhang",
      "orcid": "0000-0003-3100-8593"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/888ny9fhsg0fq.bib",
  "cited_by": 0,
  "doi": "10.1002/advs.77190",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13474175/",
  "id": "888ny9fhsg0fq",
  "issns": [
    "2198-3844"
  ],
  "landing_url": "https://doi.org/10.1002/advs.77190",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1307|Biotin and Related Studies"
    ]
  ],
  "openalex": "W7203470581",
  "pdf_url": null,
  "pmcid": "PMC13474175",
  "pmid": "42598741",
  "published": "2026-08-14",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/888ny9fhsg0fq.ris",
  "sources": [
    {
      "first_seen": "2026-11-06T12:23:13Z",
      "native_id": "PMC13474175",
      "source": "pmc",
      "url": "https://doi.org/10.1002/advs.77190"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Biotin and Related Studies",
      "subfield": "cell-biology"
    }
  ],
  "title": "Dual‐Organelle Secretome Conjugation and Organ‐Uptake Tracking (DuO‐SCOUT) Enables Deep and Sensitive Mapping of the Secreted Proteins",
  "title_text": "Dual‐Organelle Secretome Conjugation and Organ‐Uptake Tracking (DuO‐SCOUT) Enables Deep and Sensitive Mapping of the Secreted Proteins",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:20:32Z",
  "url": "https://research.draconitic.com/paper/888ny9fhsg0fq",
  "venue": "Advanced Science",
  "versions": []
}