{
  "abstract": "Cells achieve metabolic precision by assembling enzymes into dynamic complexes, but the regulatory mechanism of these metabolons is unclear. Here, we characterize the chalcone synthase (CHS)–chalcone isomerase-like protein (CHIL) complex, a key component of flavonoid metabolons. Whereas crystallography provides a static view, our analyses reveal that CHS undergoes rapid, reversible binding cycles with CHIL that regulate catalysis in real time. CHIL removes coenzyme A, an inhibitor of Claisen cyclization, transiently reshapes the CHS active site, and guides the tetraketide intermediate toward productive cyclization, thereby suppressing derailment byproducts. These findings demonstrate a previously unproven and generalizable regulatory effect in metabolons: guided active-site tuning via transient enzyme association. This concept enhances our understanding of metabolon function and opens new avenues for synthetic biology and metabolic engineering.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School of Engineering, Tohoku University, Sendai, Japan",
    "Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Japan",
    "Institute of Advanced Medical Sciences, Tokushima University, Tokushima, Japan",
    "Graduate School of Life Science, University of Hyogo, Ako-gun, Japan",
    "RIKEN SPring-8 Center, Sayo-gun, Japan",
    "Institute of Science and Engineering, Kanazawa University, Kanazawa, Japan",
    "Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan",
    "Nano Life Science Institute, Kanazawa University, Kanazawa, Japan",
    "Graduate School of Life Sciences, Tohoku University, Sendai, Japan",
    "Japan Synchrotron Radiation Research Institute (JASRI), Sayo-gun, Japan"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 30,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Riki Imaizumi",
      "orcid": "0000-0002-9578-2513"
    },
    {
      "affs": [
        0
      ],
      "name": "Toshiyuki Waki",
      "orcid": "0000-0002-0128-096X"
    },
    {
      "affs": [
        2
      ],
      "name": "Yoshikazu Hattori",
      "orcid": "0000-0002-0013-5584"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Kohei Takeshita",
      "orcid": "0000-0003-3469-9844"
    },
    {
      "affs": [
        1
      ],
      "name": "Miru Sumita",
      "orcid": "0009-0002-6300-1038"
    },
    {
      "affs": [
        5
      ],
      "name": "Kazutomo Kawaguchi",
      "orcid": "0000-0002-3387-2492"
    },
    {
      "affs": [
        6
      ],
      "name": "Hiroyuki Kumeta",
      "orcid": "0000-0003-3713-2122"
    },
    {
      "affs": [
        7
      ],
      "name": "Kenichi Umeda",
      "orcid": "0000-0002-3650-3828"
    },
    {
      "affs": [
        8
      ],
      "name": "Kyohei Sato"
    },
    {
      "affs": [
        1
      ],
      "name": "Taro Yanai",
      "orcid": "0009-0001-2019-3271"
    },
    {
      "affs": [
        3
      ],
      "name": "Hiroaki Matsuura"
    },
    {
      "affs": [
        8
      ],
      "name": "Takeshi Yokoyama",
      "orcid": "0000-0002-0299-9140"
    },
    {
      "affs": [
        7
      ],
      "name": "Risa Omura",
      "orcid": "0009-0001-5758-2051"
    },
    {
      "affs": [
        7
      ],
      "name": "Kayo Nakatani"
    },
    {
      "affs": [
        9
      ],
      "name": "Naoki Sakai",
      "orcid": "0000-0001-8544-1187"
    },
    {
      "affs": [
        0
      ],
      "name": "Yukimura Kawagiwa"
    },
    {
      "affs": [
        0
      ],
      "name": "Yamato Doi"
    },
    {
      "affs": [
        1
      ],
      "name": "AOI YASUDA"
    },
    {
      "affs": [
        0
      ],
      "name": "拓哉 中野"
    },
    {
      "affs": [
        0
      ],
      "name": "Kaichi Uno"
    },
    {
      "affs": [
        1
      ],
      "name": "Kunihiro Yoshida",
      "orcid": "0000-0001-5628-8478"
    },
    {
      "affs": [
        0
      ],
      "name": "Misato Tsunashima"
    },
    {
      "affs": [
        2
      ],
      "name": "Tomohide Saio",
      "orcid": "0000-0003-3639-7399"
    },
    {
      "affs": [
        8
      ],
      "name": "Yoshikazu Tanaka",
      "orcid": "0000-0002-7893-3236"
    },
    {
      "affs": [
        0
      ],
      "name": "Seiji Takahashi",
      "orcid": "0000-0002-2288-4340"
    },
    {
      "affs": [
        7
      ],
      "name": "Noriyuki Kodera",
      "orcid": "0000-0003-4880-8423"
    },
    {
      "affs": [
        1
      ],
      "name": "Kunishige Kataoka",
      "orcid": "0000-0001-7096-7565"
    },
    {
      "affs": [
        4
      ],
      "name": "Masaki Yamamoto",
      "orcid": "0000-0002-1311-1768"
    },
    {
      "affs": [
        1
      ],
      "name": "Satoshi Yamashita",
      "orcid": "0000-0003-2978-3216"
    },
    {
      "affs": [
        0
      ],
      "name": "Tôru Nakayama",
      "orcid": "0000-0002-5275-4731"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/88jztnh278cyf.bib",
  "cited_by": 10,
  "doi": "10.1038/s41929-026-01551-6",
  "fulltext_url": null,
  "id": "88jztnh278cyf",
  "issns": [
    "2520-1158"
  ],
  "landing_url": "https://doi.org/10.1038/s41929-026-01551-6",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Plant Gene Expression Analysis"
    ]
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  "openalex": "W7164836963",
  "pdf_url": "https://www.nature.com/articles/s41929-026-01551-6.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-06-15",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/88jztnh278cyf.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T13:21:07Z",
      "native_id": "W7164836963",
      "source": "openalex",
      "url": "https://doi.org/10.1038/s41929-026-01551-6"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Plant Gene Expression Analysis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Transient dynamics of flavonoid metabolons tune chalcone synthase specificity",
  "title_text": "Transient dynamics of flavonoid metabolons tune chalcone synthase specificity",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:07Z",
  "url": "https://research.draconitic.com/paper/88jztnh278cyf",
  "venue": "Nature Catalysis",
  "versions": []
}