{
  "abstract": "Therapy-induced senescence is increasingly recognized as a critical determinant of chemotherapy efficacy, tissue toxicity, and senolytic intervention. However, conventional senescence-associated β-galactosidase (SA-β-Gal) assays and single β-Gal-responsive probes often suffer from limited specificity because elevated β-Gal activity is not exclusive to senescent cells. Here, we report ECD-Gal, a dual-locked near-infrared fluorescent sensor that integrates β-Gal enzymatic activity with lysosomal pH variation for high-fidelity sensing therapy-induced senescence. ECD-Gal shows weak emission in β-Gal positive cells with an acidic lysosomal microenvironment but is strongly activated in senescent cells where β-Gal accumulation coincides with lysosomal alkalinization. Mechanistic studies using agents that induced lysosomal acidification or lysosomal alkalinization supported that both β-Gal activity and elevated lysosomal pH are required for fluorescence activation. ECD-Gal enabled confocal imaging, flow-cytometric quantification, in vivo visualization of doxorubicin or bleomycin-induced senescence, and its fluorescence readout correlated with SA-β-Gal staining, senescence marker expression, and fibrosis-associated pathological remodeling. This dual-locked sensing strategy provides a robust molecular tool for monitoring therapy-induced senescence and evaluating senolytic interventions in living systems.",
  "abstract_shown": true,
  "affiliations": [
    "School of Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lele Ding"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuxuan Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Na Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Zijian Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijun Jia",
      "orcid": "0000-0001-9902-8246"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/89yt6y10gqjdz.bib",
  "cited_by": 0,
  "doi": "10.1021/jacsau.6c01028",
  "fulltext_url": "https://europepmc.org/articles/PMC13714839",
  "id": "89yt6y10gqjdz",
  "issns": [
    "2691-3704"
  ],
  "landing_url": "https://europepmc.org/article/MED/42953165",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2737|Telomeres, Telomerase, and Senescence"
    ]
  ],
  "openalex": "W7213999109",
  "pdf_url": "https://europepmc.org/articles/PMC13714839?pdf=render",
  "pmcid": "PMC13714839",
  "pmid": "42953165",
  "published": "2026-09-22",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/89yt6y10gqjdz.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:41Z",
      "native_id": "MED:42953165",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42953165"
    },
    {
      "first_seen": "2026-11-01T12:22:15Z",
      "native_id": "PMC13714839",
      "source": "pmc",
      "url": "https://doi.org/10.1021/jacsau.6c01028"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2737|Telomeres, Telomerase, and Senescence",
      "subfield": "physiology"
    }
  ],
  "title": "A Dual-Locked β‑Galactosidase/pH Near-Infrared Sensor Enables High-Fidelity Monitoring of Therapy-Induced Senescence.",
  "title_text": "A Dual-Locked β‑Galactosidase/pH Near-Infrared Sensor Enables High-Fidelity Monitoring of Therapy-Induced Senescence.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:22:15Z",
  "url": "https://research.draconitic.com/paper/89yt6y10gqjdz",
  "venue": "JACS Au",
  "versions": []
}