{
  "abstract": "The traditional synthesis of nucleotide 5'-5' triphosphate linkages involves a \"2 + 1\" phosphate coupling mediated by a large excess of metal salts, where the salt removal is a labor and waste-intensive step. We have omitted the metal salts from this reaction by modulating the solubility and reactivity of the monophosphate coupling partner using a precise combination of the reaction solvent and the paired cation. This resulted in simplified downstream purification, where the amount of waste generated was reduced by over 60% throughout the whole process. We have demonstrated this synthetic approach on ARCA, an mRNA 5'-capping agent. Both the chemical purity of the product and its performance in mRNA was comparable to commercial samples.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Sustainability for Chemicals, Energy and Environment (ISCE2), Agency for Science, Technology and Research (A*STAR) 8 Biomedical Grove, #07-01 Neuros Building Singapore 138665 Republic of Singapore lim_yee_hwee@a-star.edu.sg.",
    "Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR) 60 Biopolis Street, #02-01 Genome Building Singapore 138672 Republic of Singapore.",
    "Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR) 20 Biopolis Way, #06-01 Centros Building Singapore 138668 Republic of Singapore."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Eric Tam"
    },
    {
      "affs": [
        0
      ],
      "name": "Viktor Barát"
    },
    {
      "affs": [
        1
      ],
      "name": "Tzuen Yih Saw"
    },
    {
      "affs": [
        1
      ],
      "name": "Kuo-Chieh Liao"
    },
    {
      "affs": [
        1
      ],
      "name": "Yue Wan"
    },
    {
      "affs": [
        2
      ],
      "name": "Shuwen Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Ying Swan Ho"
    },
    {
      "affs": [
        0
      ],
      "name": "Yee Hwee Lim",
      "orcid": "0000-0002-7789-3893"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8ak4zmmrj11wv.bib",
  "cited_by": 0,
  "doi": "10.1039/d6ra08498b",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13717511/?tool=EBI",
  "id": "8ak4zmmrj11wv",
  "issns": [
    "2046-2069"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13717511",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Chemistry"
    ],
    [
      "openalex",
      "1312|DNA and Nucleic Acid Chemistry"
    ]
  ],
  "openalex": "W7224182446",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13717511/pdf/?tool=EBI",
  "pmcid": "PMC13717511",
  "pmid": "42959525",
  "published": "2026-10-29",
  "publisher": "Royal Society of Chemistry",
  "ris": "https://research.draconitic.com/paper/8ak4zmmrj11wv.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:37Z",
      "native_id": "PMC:PMC13717511",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13717511"
    },
    {
      "first_seen": "2026-11-01T00:20:50Z",
      "native_id": "MED:42959525",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42959525"
    },
    {
      "first_seen": "2026-11-02T12:21:49Z",
      "native_id": "PMC13717511",
      "source": "pmc",
      "url": "https://doi.org/10.1039/d6ra08498b"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|DNA and Nucleic Acid Chemistry",
      "subfield": "molecular-biology"
    }
  ],
  "title": "An activator-free method for the synthesis of nucleotide 5′–5′ triphosphate mRNA capping agent",
  "title_text": "An activator-free method for the synthesis of nucleotide 5′–5′ triphosphate mRNA capping agent",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:21:49Z",
  "url": "https://research.draconitic.com/paper/8ak4zmmrj11wv",
  "venue": "RSC advances",
  "versions": []
}