{
  "abstract": "We report a generalizable method for single-molecule localization super-resolution microscopy in living cells. Live-cell PAINT (points accumulation for imaging in nanoscale topography) and single-molecule diffusivity mapping (SMdM) of intracellular protein targets are achieved by pairing exceptionally fluorogenic bis-trifluoromethyl rhodamine (BF) dyes with reversible HaloTag ligands and widely available HaloTag fusion proteins. This far-red small-molecule update to protein-based PAINT is readily incorporated into existing super-resolution microscopy workflows: wash-free staining and imaging are achieved for cell lines and primary neuron cultures for diverse protein targets. Pairing with photoconvertible fluorescent proteins further enables simultaneous two-color live-cell super-resolution microscopy and SMdM.",
  "abstract_shown": true,
  "affiliations": [
    "University of California, Berkeley"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "T. Zhan"
    },
    {
      "name": "N. C. Gerstner"
    },
    {
      "name": "J. G. Martin"
    },
    {
      "name": "J. T. McCann"
    },
    {
      "name": "M. C. Lynch"
    },
    {
      "name": "H.-J. Wu"
    },
    {
      "name": "K. Xu"
    },
    {
      "affs": [
        0
      ],
      "name": "E. W. Miller"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8btd66qw4f0xz.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.07.16.738981",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.07.16.738981v2.full",
  "id": "8btd66qw4f0xz",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.07.16.738981v2",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7169627233",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.07.16.738981v2.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-16",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8btd66qw4f0xz.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:25Z",
      "native_id": "10.64898/2026.07.16.738981",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.07.16.738981v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "Live-cell PAINT microscopy of protein targets via fluorogenic exchangeable HaloTag ligands",
  "title_text": "Live-cell PAINT microscopy of protein targets via fluorogenic exchangeable HaloTag ligands",
  "type": "preprint",
  "updated_at": "2026-10-30T12:21:25Z",
  "url": "https://research.draconitic.com/paper/8btd66qw4f0xz",
  "venue": "bioRxiv",
  "versions": []
}