{
  "abstract": "Respiratory syncytial virus (RSV) is a major cause of severe respiratory infections worldwide, particularly in infants, young children, and elderly individuals. Despite the availability of preventive options such as vaccines and monoclonal antibodies, effective antiviral therapies for acute infections remain limited. Therefore, we investigated small-molecule entry inhibitors identified in a high-throughput campaing and evaluated the most promising candidates in detail by performing resynthesis and determining their antiviral activity. Unfortunately, analytical investigations by nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS) revealed discrepancies between the screening samples and the resynthesized compounds for two compound classes, highlighting the complexity of hit validation and the potential influence of impurities. By publishing these results, we aim not only to underscore the importance of rigorous analytical and biological validation in screening campaigns but also to support the development of improved strategies for identifying reliable RSV inhibitors. We also believe it is important to publish false-positive hits to avoid time efforts on projects that assume such compounds as genuine hits.",
  "abstract_shown": true,
  "affiliations": [
    "Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Department of Pharmacy, Saarland University, Saarbrücken, Germany.",
    "TWINCORE, Centre for Experimental and Clinical Infection Research, A Joint Venture Between the Helmholtz Centre for Infection Research and the Hannover Medical School, Hannover, Germany.",
    "PharmaScienceHub (PSH), Saarland University, Saarbrücken, Germany.",
    "Medizinische Hochschule Hannover-Institut für Virologie, MHH, Hannover, Germany.",
    "Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.",
    "German Centre for Infection Research, Partner Site Hamburg-Borstel-Lübeck-Riems, Hamburg, Germany.",
    "Institute for Molecular Virology and Tumorvirology, University Medical Center Hamburg Eppendorf, Hamburg, Germany.",
    "German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover, (BREATH), Hannover, Germany.",
    "Cluster of Excellence RESIST (EXC 2155), Hanover Medical School, Hannover, Germany."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Christina Karhan"
    },
    {
      "affs": [
        1
      ],
      "name": "Svenja M Sake"
    },
    {
      "affs": [
        1
      ],
      "name": "Melanie Urbanek-Quaing"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Diletta Bergamo"
    },
    {
      "affs": [
        3
      ],
      "name": "Jessica Rückert"
    },
    {
      "affs": [
        4
      ],
      "name": "Jiabin Huang",
      "orcid": "0000-0002-3480-7115"
    },
    {
      "affs": [
        1
      ],
      "name": "Christina Grethe"
    },
    {
      "affs": [
        1
      ],
      "name": "Antonia P Gunesch"
    },
    {
      "affs": [
        1
      ],
      "name": "Anne Kühnel"
    },
    {
      "affs": [
        1
      ],
      "name": "Birthe Reinecke",
      "orcid": "0000-0003-3875-0707"
    },
    {
      "affs": [
        5,
        6
      ],
      "name": "Nicole Fischer"
    },
    {
      "affs": [
        3,
        7,
        8
      ],
      "name": "Thomas F Schulz"
    },
    {
      "affs": [
        1,
        7,
        8
      ],
      "name": "Thomas Pietschmann",
      "orcid": "0000-0001-6789-4422"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Anna K H Hirsch",
      "orcid": "0000-0001-8734-4663"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8ckvyk7rpqg6d.bib",
  "cited_by": 0,
  "doi": "10.1002/cmdc.70501",
  "fulltext_url": "https://europepmc.org/articles/PMC13687780",
  "id": "8ckvyk7rpqg6d",
  "issns": [
    "1860-7179",
    "1860-7187"
  ],
  "landing_url": "https://europepmc.org/article/MED/42908255",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2713|Respiratory viral infections research"
    ]
  ],
  "openalex": "W7223169097",
  "pdf_url": "https://europepmc.org/articles/PMC13687780?pdf=render",
  "pmcid": "PMC13687780",
  "pmid": "42908255",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8ckvyk7rpqg6d.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:57Z",
      "native_id": "MED:42908255",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42908255"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Respiratory viral infections research",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Validation of Small-Molecule Entry Inhibitors Targeting the Respiratory Syncytial Virus.",
  "title_text": "Validation of Small-Molecule Entry Inhibitors Targeting the Respiratory Syncytial Virus.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:57Z",
  "url": "https://research.draconitic.com/paper/8ckvyk7rpqg6d",
  "venue": "ChemMedChem",
  "versions": []
}