{
  "abstract": "Genome-edited human pluripotent stem cells (hPSCs) provide a powerful platform to study complex diseases such as Parkinson’s disease (PD). Here, we describe iSCORE-PD, an isogenic collection of 65 genome-edited hPSC lines carrying disease-causing or high-risk variants in 11 PD-linked genes ( SNCA, PRKN, PINK1, DJ1/PARK7, LRRK2, ATP13A2, FBXO7, DNAJC6, SYNJ1, VPS13C , and GBA1 ). All lines are derived from a well-characterized female hESC line and subjected to extensive quality control. Whole-genome sequencing reveals that genetic variation between lines, largely confined to non-coding regions, is minimal relative to inter-individual differences in patient-derived hiPSCs, with most variation arising from random mutations acquired during cell culture rather than genome-editing-induced off-target effects. Including multiple independently derived clones per mutation can control for this random genetic drift. Our systematic approach ensures high quality of this publicly available iSCORE-PD resource, highlights the advantages of prime editing over conventional CRISPR/Cas9 methods, and establishes best practices for generating disease-modeling hPSC collections.",
  "abstract_shown": true,
  "affiliations": [
    "Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, USA",
    "Dominick P. Purpura Department of Neuroscience, Rose F. Kennedy Center, Albert Einstein College of Medicine, Bronx, NY, USA",
    "Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, NY, USA",
    "Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815, USA",
    "Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Rose F. Kennedy Center, Albert Einstein College of Medicine, 40 Pelham Parkway South, Bronx, NY 046, USA",
    "Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, 1301 Morris Park Ave., Bronx, NY 10461, USA",
    "Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA",
    "Innovative Genomics Institute, University of California, Berkeley, CA, USA",
    "Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 9720, USA",
    "Center for Alzheimer's and Related Dementias, National Institute on Aging and National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA",
    "Department of Neurodegenerative Disease, UCL Queen Square Institute of Neurology, University College London, London, UK",
    "Center for Alzheimer's and Related Dementias, National Institute on Aging and National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 20892, USA",
    "Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA",
    "Department of Genetics, Albert Einstein College of Medicine, 1301 Morris Park Ave., Bronx, NY 10461, USA",
    "Department of Neuroscience, University of California, Berkeley, Berkeley, CA, USA",
    "Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD, USA",
    "Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD, 20892, USA",
    "LiftOff Biosolution, Santa Cruz, CA, USA",
    "Chan Zuckerberg Biohub, San Francisco, CA, 4158, USA",
    "Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA",
    "Chan Zuckerberg Biohub, San Francisco, CA, USA",
    "Innovative Genomics Institute, University of California, Berkeley, CA, 94720, USA",
    "Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, USA. hockemeyer@berkeley.edu",
    "Chan Zuckerberg Biohub, San Francisco, CA, USA. hockemeyer@berkeley.edu",
    "Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. hockemeyer@berkeley.edu",
    "Innovative Genomics Institute, University of California, Berkeley, CA, USA. hockemeyer@berkeley.edu",
    "Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, USA. frank.soldner@einsteinmed.edu",
    "Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA. frank.soldner@einsteinmed.edu",
    "Dominick P. Purpura Department of Neuroscience, Rose F. Kennedy Center, Albert Einstein College of Medicine, Bronx, NY, USA. frank.soldner@einsteinmed.edu",
    "Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, NY, USA. frank.soldner@einsteinmed.edu"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 28,
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      "name": "Oriol Busquets",
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      "name": "Hanqin Li",
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      "name": "Vivien M. Simon",
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      "name": "Zipporiah Bush",
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      "name": "Jessica Diaz",
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      "name": "Atehsa Sahagun",
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      "name": "Jianpu Gao",
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      "name": "Samantha Hong",
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      "name": "Dena G. Hernandez",
      "orcid": "0000-0002-0188-9927"
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      "name": "Kristin S. Levine",
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      "name": "Nathalie L.M.M. Pochet",
      "orcid": "0000-0003-1820-5656"
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      "name": "Ezgi O. Booth",
      "orcid": "0000-0001-9213-8426"
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      "name": "Marco Blanchette",
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      "name": "Helen S. Bateup",
      "orcid": "0000-0002-0135-0972"
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      "name": "Donald C. Rio",
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      "name": "Cornelis Blauwendraat",
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      "name": "Dirk F. Hockemeyer",
      "orcid": "0000-0002-5598-5092"
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      "name": "Frank Soldner",
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  "bibtex": "https://research.draconitic.com/paper/8d06cdn4qrgxj.bib",
  "cited_by": 9,
  "doi": "10.1038/s41467-026-74355-8",
  "fulltext_url": null,
  "id": "8d06cdn4qrgxj",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://doi.org/10.1038/s41467-026-74355-8",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "1312|Pluripotent Stem Cells Research"
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  "openalex": "W4391820161",
  "pdf_url": null,
  "pmcid": "PMC10888955",
  "pmid": "38405931",
  "published": "2026-06-17",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/8d06cdn4qrgxj.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:48Z",
      "native_id": "W4391820161",
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      "url": "https://doi.org/10.1038/s41467-026-74355-8"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Pluripotent Stem Cells Research",
      "subfield": "molecular-biology"
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  ],
  "title": "iSCORE-PD: an isogenic stem cell collection to research Parkinson’s disease",
  "title_text": "iSCORE-PD: an isogenic stem cell collection to research Parkinson’s disease",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T13:20:43Z",
  "url": "https://research.draconitic.com/paper/8d06cdn4qrgxj",
  "venue": "Nature Communications",
  "versions": []
}