{
  "abstract": "Reversibly photoswitchable fluorophores have enabled a broad range of applications in advanced fluorescence bioimaging. Here, we introduce RSpFAST, a new class of reversibly photoswitchable fluorescent labels that combine a biomolecular host (pFAST protein tag) with a reversibly photoisomerizable guest (fluorogen), allowing fluorescence brightness to be modulated through illumination and molecular complexation. We combine thermokinetic, photochemical, and structural investigations to obtain a comprehensive mechanistic and kinetic understanding of RSpFAST. Building on this theoretical framework, we demonstrate in both live and fixed cells that RSpFAST exhibits an unprecedented dual behavior: a stable and wash-free fluorescent labeling tag turns into a negative reversible photoswitcher by decreasing the fluorogen concentration and increasing light intensity. In this photoejection-driven kinetic regime, RSpFAST is shown to be an efficient marker for dynamic contrast and super-resolution microscopy.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 23,
  "authors": [
    {
      "name": "Yuriy Shpinov"
    },
    {
      "name": "Mrinal Mandal"
    },
    {
      "name": "Vincent van Deuren"
    },
    {
      "name": "Alienor Lahlou"
    },
    {
      "name": "Matthias Le Bec"
    },
    {
      "name": "Raja Chouket"
    },
    {
      "name": "Chaima Hadj Moussa"
    },
    {
      "name": "Clara Bonin"
    },
    {
      "name": "Hessam Sepasi Tehrani"
    },
    {
      "name": "Ian Coghill"
    },
    {
      "name": "Lina El Hajji"
    },
    {
      "name": "Karim Ounoughi"
    },
    {
      "name": "Jaime Franco Pinto"
    },
    {
      "name": "Marie-Aude Plamont"
    },
    {
      "name": "Philippe Pelupessy"
    },
    {
      "name": "Isabel Ayala"
    },
    {
      "name": "Franck Perez"
    },
    {
      "name": "Isabelle Aujard"
    },
    {
      "name": "Thomas Le Saux"
    },
    {
      "name": "Arnaud Gautier"
    },
    {
      "name": "Peter Dedecker"
    },
    {
      "name": "Bernhard Brutscher"
    },
    {
      "name": "Ludovic Jullien"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8dajnmfcc1gk1.bib",
  "cited_by": 0,
  "doi": "10.1021/jacs.6c08277",
  "fulltext_url": null,
  "id": "8dajnmfcc1gk1",
  "issns": [
    "0002-7863",
    "1520-5126"
  ],
  "landing_url": "https://hal.science/hal-05803513v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.chim"
    ],
    [
      "openalex",
      "2505|Photochromic and Fluorescence Chemistry"
    ]
  ],
  "openalex": "W7204845612",
  "pdf_url": "https://hal.science/hal-05803513/document",
  "pmcid": null,
  "pmid": "42714498",
  "published": "2026-08-25",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/8dajnmfcc1gk1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:58Z",
      "native_id": "hal-05803513",
      "source": "hal",
      "url": "https://hal.science/hal-05803513v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2505|Photochromic and Fluorescence Chemistry",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Kinetic and Mechanistic Characterization of RSpFAST, a Non-Covalent Versatile Chemogenetic Platform for Fluorescence Imaging",
  "title_text": "Kinetic and Mechanistic Characterization of RSpFAST, a Non-Covalent Versatile Chemogenetic Platform for Fluorescence Imaging",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:58Z",
  "url": "https://research.draconitic.com/paper/8dajnmfcc1gk1",
  "venue": "Journal of the American Chemical Society",
  "versions": []
}