{
  "abstract": "Enterocytes and intestinal secretory cells harbor innate immunity. However, certain transcriptional programs -even beyond rearranged T- and B-cell receptor genes- are considered the exclusive purview of adaptive immune cells, including abundant and diverse intestinal intra-epithelial lymphocytes (IELs). Here, we identify and characterize columnar intestinal secretory cells that co-express classic epithelial and lymphoid genes, including Ptprc, Cd3e, Cd8a, and Gzma/b but not T- or B-cell receptors. These previously unrecognized epithelium-derived lymphoid-like cells (EDLCs) differ from any known resident cell type, including IELs or tuft cells. Rare in healthy mouse and human intestines, their numbers increase upon IL-25 stimulation or IL-10 receptor deficiency in vivo and upon IL-13 stimulation in vitro. EDLC chromatin is uniquely accessible at thousands of cis-elements, including lymphoid and epithelial loci. These findings reveal a transcriptionally and epigenetically distinct intestinal epithelial derivative that expresses prominent lymphoid character.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Medical Oncology and Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA 02215, USA.",
    "Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.",
    "Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.",
    "Graduate Program in Genetics, State University of New York, Stony Brook, NY 11794, USA.",
    "Division of Gastroenterology, Hepatology, and Nutrition, Boston Children’s Hospital, Boston, MA 02115, USA.",
    "Section of Surgical Sciences, Epithelial Biology Center, and Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, and Nashville VA Medical Center, Nashville, TN 37212, USA.",
    "Molecular Imaging Core and Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.",
    "Harvard Stem Cell Institute, Cambridge, MA 02138, USA."
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Swarnabh Bhattacharya",
      "orcid": "0000-0003-0354-2350"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Krithika Badarinath"
    },
    {
      "affs": [
        0
      ],
      "name": "Guodong Tie"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Pratik N. P. Singh"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Onur Eskiocak"
    },
    {
      "affs": [
        0
      ],
      "name": "Ruiyang He"
    },
    {
      "affs": [
        4
      ],
      "name": "Bin Bao",
      "orcid": "0000-0001-7610-3426"
    },
    {
      "affs": [
        5
      ],
      "name": "Su-Hyung Lee"
    },
    {
      "affs": [
        1,
        4
      ],
      "name": "Scott Snapper"
    },
    {
      "affs": [
        6
      ],
      "name": "Kun Huang"
    },
    {
      "affs": [
        5
      ],
      "name": "James R. Goldenring"
    },
    {
      "affs": [
        2
      ],
      "name": "Semir Beyaz",
      "orcid": "0000-0003-4730-4012"
    },
    {
      "affs": [
        0,
        1,
        7
      ],
      "name": "Ramesh A. Shivdasani",
      "orcid": "0000-0002-2828-1727"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8e0v7zsmd5216.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.21.760658",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760658v1.full",
  "id": "8e0v7zsmd5216",
  "issns": [
    "2692-8205"
  ],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760658v1",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cell biology"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2403|IL-33, ST2, and ILC Pathways"
    ]
  ],
  "openalex": "W7223674423",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760658v1.full.pdf",
  "pmcid": "PMC13733712",
  "pmid": "42990162",
  "published": "2026-10-21",
  "publisher": "Cold Spring Harbor Laboratory Preprints",
  "ris": "https://research.draconitic.com/paper/8e0v7zsmd5216.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:39Z",
      "native_id": "10.64898/2026.10.21.760658",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760658v1"
    },
    {
      "first_seen": "2026-11-08T12:23:25Z",
      "native_id": "PMC13733712",
      "source": "pmc",
      "url": "https://doi.org/10.64898/2026.10.21.760658"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:cell biology",
      "subfield": "cell-biology"
    }
  ],
  "title": "A unique intestinal epithelial cell that expresses canonical lymphoid genes",
  "title_text": "A unique intestinal epithelial cell that expresses canonical lymphoid genes",
  "type": "preprint",
  "updated_at": "2026-11-08T12:23:25Z",
  "url": "https://research.draconitic.com/paper/8e0v7zsmd5216",
  "venue": "bioRxiv",
  "versions": []
}