{ "abstract": "Background: Focal liver lesions (FLLs) encompass a wide spectrum of benign and malignant pathologies, and accurate diagnosis is essential for appropriate management. Although advances in imaging have improved lesion characterization, histopathologic assessment remains the diagnostic gold standard for indeterminate lesions. Data on the histopathologic spectrum and diagnostic utility of ultrasound-guided percutaneous liver biopsy (US-PLB) in sub-Saharan Africa (SSA) are limited. This study aimed to characterize the histopathologic findings of US-PLB performed for FLLs at a tertiary referral center in SSA and to identify factors associated with hepatocellular carcinoma (HCC). Methods: We conducted a retrospective observational study of adult patients (≥18 years) who underwent US-PLB for FLL between January 2021 and December 2024 at Adera Medical and Surgical Center. Patients with indeterminate pathology results, incomplete records, biopsies performed for diffuse liver disease, or lesions classified as LI-RADS 1, 2, or 5 were excluded. Demographic, clinical, laboratory, imaging, histopathologic, and outcome data were extracted from medical records. Descriptive statistics were used to summarize patient characteristics and histopathologic diagnoses. Logistic regression analysis was performed to identify factors associated with HCC. Results: A total of 119 were included in the final analysis. The median age was 56 years (IQR 45–65), and 59.7% were male. The median focal liver lesion size was 8.20 cm (IQR: 5.1–11.0 cm), with 38.7% presenting as multifocal lesions. No major biopsy-related complications were reported. HCC was the most common histopathologic diagnosis, accounting for 42.9% of cases, followed by secondary metastatic tumors (15.9%) and regenerative nodules (15.9%). Other diagnoses included chronic hepatitis (8.4%), cholangiocarcinoma (5.9%), and hepatic abscess (3.4%). Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections were present in 14.3% and 12.4% of patients, respectively. On multivariate analysis, HBV infection (AOR 7.85, 95% CI 1.45–42.60; p = 0.017), HCV infection (AOR 9.03, 95% CI 1.41–57.76; p = 0.020), Fibrosis-4 (FIB-4) score (AOR 1.45, 95% CI: 1.05–2.34: p < 0.01), and larger tumor size (AOR 1.27, 95% CI 1.11–1.46; p < 0.01) were significantly associated with HCC. Conclusion: Ultrasound-guided percutaneous liver biopsy demonstrated a favorable safety profile for the evaluation of FLL. HCC was the predominant histopathologic diagnosis, reflecting the substantial burden of primary liver cancer in this setting. Chronic viral hepatitis and larger tumor size were significantly associated with HCC. These findings support the continued role of US-PLB in the diagnostic evaluation of indeterminate focal liver lesions and underscores the importance of viral hepatitis prevention, surveillance, and early detection strategies in sub-Saharan Africa.", "abstract_shown": true, "affiliations": [ "Department of Internal Medicine, University of Rwanda, Kigali, Rwanda", "Department of Internal Medicine, Adera Medical and Surgical Center, Addis Ababa, Ethiopia", "Department of Internal Medicine, Addis Ababa University, Addis Ababa, Ethiopia", "Department of Internal Medicine, Dilla University, Dilla, Ethiopia", "Department of Internal Medicine, St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia" ], "article_type": "Research Article", "arxiv": null, "author_total": 7, "authors": [ { "affs": [ 0 ], "name": "Tamrat Petros Elias" }, { "affs": [ 1 ], "name": "Abate Bane Shewaye" }, { "affs": [ 2 ], "name": "Kaleb Assefa Berhane" }, { "affs": [ 3 ], "name": "Abdu Mohammed" }, { "affs": [ 4 ], "name": "Zebeaman Tibebu" }, { "name": "Amanuel Getu Gebreselassie" }, { "name": "Abraham Sisay Abie" } ], "bibtex": "https://research.draconitic.com/paper/8em8jh3mrdbgn.bib", "cited_by": 0, "doi": "10.1371/journal.pone.0357210", "fulltext_url": null, "id": "8em8jh3mrdbgn", "issns": [ "1932-6203" ], "landing_url": "https://doi.org/10.1371/journal.pone.0357210", "language": null, "licence": "CC-BY-4.0", "licence_label": "CC BY 4.0", "licence_source": "journal", "licence_url": "https://creativecommons.org/licenses/by/4.0/", "metadata_licence": null, "native_subjects": [ [ "openalex", "2721|Hepatocellular Carcinoma Treatment and Prognosis" ], [ "plos", "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Viral pathogens/Flaviviruses/Hepacivirus/Hepatitis C virus" ], [ "plos", "/Biology and life sciences/Microbiology/Medical microbiology/Microbial pathogens/Viral pathogens/Hepatitis viruses/Hepatitis C virus" ], [ "plos", "/Biology and life sciences/Organisms/Viruses/RNA viruses/Flaviviruses/Hepacivirus/Hepatitis C virus" ], [ "plos", "/Biology and life sciences/Organisms/Viruses/Viral pathogens/Flaviviruses/Hepacivirus/Hepatitis C virus" ], [ "plos", "/Biology and life sciences/Organisms/Viruses/Viral pathogens/Hepatitis viruses/Hepatitis C virus" ], [ "plos", "/Medicine and health sciences/Clinical medicine/Signs and symptoms/Lesions" ], [ "plos", "/Medicine and health sciences/Diagnostic medicine/Cancer detection and diagnosis" ], [ "plos", "/Medicine and health sciences/Diagnostic medicine/Diagnostic radiology/Gastrointestinal imaging/Liver and spleen scan" ], [ "plos", "/Medicine and health sciences/Gastroenterology and hepatology/Liver diseases/Chronic liver disease/Chronic hepatitis" ], [ "plos", "/Medicine and health sciences/Gastroenterology and hepatology/Liver diseases/Cirrhosis" ], [ "plos", "/Medicine and health sciences/Gastroenterology and hepatology/Liver diseases/Hepatocellular carcinoma" ], [ "plos", "/Medicine and health sciences/Oncology/Cancer detection and diagnosis" ], [ "plos", "/Medicine and health sciences/Oncology/Cancers and neoplasms/Carcinoma/Hepatocellular carcinoma" ], [ "plos", "/Medicine and health sciences/Oncology/Cancers and neoplasms/Gastrointestinal tumors/Hepatocellular carcinoma" ], [ "plos", "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Viral pathogens/Flaviviruses/Hepacivirus/Hepatitis C virus" ], [ "plos", "/Medicine and health sciences/Pathology and laboratory medicine/Pathogens/Microbial pathogens/Viral pathogens/Hepatitis viruses/Hepatitis C virus" ], [ "plos", "/Medicine and health sciences/Radiology and imaging/Diagnostic radiology/Gastrointestinal imaging/Liver and spleen scan" ], [ "plos", "/Medicine and health sciences/Surgical and invasive medical procedures/Biopsy" ], [ "plos", "/Research and analysis methods/Imaging techniques/Diagnostic radiology/Gastrointestinal imaging/Liver and spleen scan" ] ], "openalex": "W7222611053", "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0357210&type=printable", "pmcid": null, "pmid": null, "published": "2026-10-16", "publisher": "Public Library of Science", "ris": "https://research.draconitic.com/paper/8em8jh3mrdbgn.ris", "sources": [ { "first_seen": "2026-10-30T12:17:23Z", "native_id": "10.1371/journal.pone.0357210", "source": "plos", "url": "https://doi.org/10.1371/journal.pone.0357210" } ], "subjects": [ { "field": "biology", "origin": "plos:microbiology", "subfield": "microbiology" }, { "field": "medicine", "origin": "plos:diagnostic radiology", "subfield": "imaging" }, { "field": "medicine", "origin": "plos:oncology", "subfield": "oncology" } ], "title": "Histopathologic spectrum of focal liver lesions diagnosed by ultrasound-guided percutaneous liver biopsy and predictors of hepatocellular carcinoma: A single-center experience from Sub-Saharan Africa", "title_text": "Histopathologic spectrum of focal liver lesions diagnosed by ultrasound-guided percutaneous liver biopsy and predictors of hepatocellular carcinoma: A single-center experience from Sub-Saharan Africa", "type": "peer-reviewed", "updated_at": "2026-10-30T12:17:23Z", "url": "https://research.draconitic.com/paper/8em8jh3mrdbgn", "venue": "PLOS One", "versions": [] }