{
  "abstract": "The human T cell repertoire is generated through recombination of a multitude of T cell receptor gene segments, yielding a complex array of T cell clones. T cell receptor beta (TRB) V gene segment defined, T cell clonal frequency when organized in correspondence with the respective V gene segment positions on the TRB genomic locus yields a periodic, undulating curve in the spatial domain. Using the genomic distance from the TRB-D1 segment to the TRB-V1-29 segments, spectral analysis was performed utilizing Lomb-Scargle periodogram (modified Fourier analysis) to obtain spectral power curves quantifying the TRB V clonal frequencies from 12 allogeneic stem cell transplant donors (baseline) and recipients (<100 days or b/w 100-365) using a variety of analytic software. Patients either underwent HLA matched related bone marrow or HLA matched unrelated blood stem cell transplantation, using PT-CY for the former and ATG for the latter. Spectral Power curves revealed dominant spectral peaks at wavelengths ranging from 113-335 millicycles/kb in the 12 donors, with consistent frequency domain spectral patterns, supporting similar use of V segments across healthy individuals. PT-CY recipients had power spectra closely corresponding to the respective donors, however the ATG recipients had relatively dispersed spectra, with spectral centroid shifted towards higher frequencies compared to donors and a reciprocal decline in the low frequency indices. Spectral power was concentrated in the <3 kb and 3-12 kb wavelengths in both groups, consistent with the periodicity observed in the relative V gene segment usage across the repertoire. The analyses reported here demonstrate that the healthy SCT donors have a spectral signature occupying short to intermediate wavelegnths in the frequency domain, PT CY recipients recover with donor-like spectra on the average, however ATG recipients tend to shift towards higher frequencies. These findings are consistent with a normal organized distribution of TRB V segment usage in healthy individuals, with PT-CY preserving this organization more consistently than ATG. Spectral (Fourier) analysis of TRB sequencing data provides a repertoire wide summary of T cell clonal distribution.",
  "abstract_shown": true,
  "affiliations": [
    "Virginia Cancer Institute & Sarah Cannon Research Institute"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "A. A. Toor"
    },
    {
      "name": "A. Marinos-Velarde"
    },
    {
      "name": "R. Qayyum"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8fdhdgz5dcmyh.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.28.26364185",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.09.28.26364185v1.full",
  "id": "8fdhdgz5dcmyh",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.09.28.26364185v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "transplantation"
    ],
    [
      "openalex",
      "2720|Hematopoietic Stem Cell Transplantation"
    ]
  ],
  "openalex": "W7218852333",
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.09.28.26364185v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-28",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8fdhdgz5dcmyh.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:39Z",
      "native_id": "10.64898/2026.09.28.26364185",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.09.28.26364185v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:transplantation",
      "subfield": "surgery"
    }
  ],
  "title": "Spectral Analysis of the T Cell Repertoire Recovery Following Allogeneic Stem Cell Transplantation",
  "title_text": "Spectral Analysis of the T Cell Repertoire Recovery Following Allogeneic Stem Cell Transplantation",
  "type": "preprint",
  "updated_at": "2026-10-30T12:19:39Z",
  "url": "https://research.draconitic.com/paper/8fdhdgz5dcmyh",
  "venue": "medRxiv",
  "versions": []
}