{
  "abstract": "Angiogenesis plays a crucial role in cancer metastasis as tumors need to establish an increased blood supply to satisfy the demand for oxygen and nutrients. Numerous genes regulate this process, and various mechanisms including microRNAs regulate the expression of these genes. In the current study, we hypothesized that miR-6756 and miR-3190 regulate angiogenic mediators such as transcription factor ETS-related gene (ERG). Detailed bioinformatics analyses were performed to locate binding sites of miR-6756 and miR-3190 on ERG mRNA. miR-6756 and miR-3190 were cloned and overexpressed in primary endothelial cells to analyze ERG expression. To evaluate ERG activity reporter assays were performed in response to miR-6756 and miR-3190 over-expression. The effects of miRs on angiogenesis were measured by cell migration rate using the scratch assay. The expression of angiogenic targets of ERG was analyzed through qPCR with and without miR-6756 and miR-3190 overexpression. Lastly, VE-cadherin; an endothelial-specific ERG target was analyzed after miRs overexpression. Bioinformatics analyses showed that both miR-6756 and miR-3190 each have three binding sites on the mRNA of ERG. ERG expression at both mRNA (p < 0.001) and the protein level (p < 0.001) was significantly reduced after miR-6756 and miR-3190 overexpression. Also, the transcriptional activity of ERG significantly reduced (p < 0.05) in response to miRs overexpression. Cell migration rate (p < 0.001) and ERG angiogenic target genes were also decreased significantly (p < 0.001). VE-cadherin also showed significantly reduced expression and activity after miR-6756 and miR-3190 over-expression. This study indicates that miR-6756 and miR-3190 can regulate ERG expression and its target genes.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Zoology, GC University, Lahore, Pakistan",
    "Department of Basic Sciences Research, Shaukat Khanum Memorial Cancer Hospital and Research Centre (SKMCH & RC), Lahore, Pakistan",
    "Moores Cancer Center, University of California, San Diego, United States of America"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sidra Mumtaz"
    },
    {
      "affs": [
        1
      ],
      "name": "Muhammad Usman Rashid"
    },
    {
      "affs": [
        2
      ],
      "name": "Safir Ullah Khan"
    },
    {
      "name": "Naila Malkani"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8fgzjn37hn2xa.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0358739",
  "fulltext_url": null,
  "id": "8fgzjn37hn2xa",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0358739",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|MicroRNA in disease regulation"
    ],
    [
      "plos",
      "/Biology and life sciences/Anatomy/Biological tissue/Epithelium/Epithelial cells/Endothelial cells"
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    [
      "plos",
      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/Messenger RNA"
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    [
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      "/Biology and life sciences/Biochemistry/Nucleic acids/RNA/Non-coding RNA/Natural antisense transcripts/MicroRNAs"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/DNA-binding proteins/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Biochemistry/Proteins/Regulatory proteins/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Epithelial cells/Endothelial cells"
    ],
    [
      "plos",
      "/Biology and life sciences/Developmental biology/Angiogenesis"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/MicroRNAs"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation/Transcription factors"
    ],
    [
      "plos",
      "/Biology and life sciences/Molecular biology/Molecular biology techniques/Transfection"
    ],
    [
      "plos",
      "/Biology and life sciences/Physiology/Cardiovascular physiology/Angiogenesis"
    ],
    [
      "plos",
      "/Medicine and health sciences/Anatomy/Biological tissue/Epithelium/Epithelial cells/Endothelial cells"
    ],
    [
      "plos",
      "/Research and analysis methods/Molecular biology techniques/Transfection"
    ]
  ],
  "openalex": "W7214290938",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0358739&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-25",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/8fgzjn37hn2xa.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:43Z",
      "native_id": "10.1371/journal.pone.0358739",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0358739"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
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    {
      "field": "medicine",
      "origin": "plos:anatomy",
      "subfield": "physiology"
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  ],
  "title": "Modulation of angiogenic transcription factor ERG via miR-6756 and miR-3190",
  "title_text": "Modulation of angiogenic transcription factor ERG via miR-6756 and miR-3190",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:43Z",
  "url": "https://research.draconitic.com/paper/8fgzjn37hn2xa",
  "venue": "PLOS One",
  "versions": []
}