{
  "abstract": "Microtubule nucleation by γ-tubulin ring complexes (γ-TuRCs) must be spatiotemporally controlled to avoid major cell division errors. γ-TuRCs are activated by the centrosome-specific binding of a highly conserved Centrosomin Motif 1 (CM1) domain, but how binding is prevented in the cytosol remains unclear. We provide the first structural explanation for CM1 autoinhibition, revealing that stable intramolecular interactions mediate cooperative folding to block γ-TuRC binding interfaces. Using various structural approaches, we show that the CM1 forms a conserved dimeric coiled-coil hairpin that sequesters a key phenylalanine residue essential for γ-TuRC binding. This appears sufficient for autoinhibition in humans, but in flies autoinhibition requires the association of an upstream “CM1 autoinhibition” (CAI) domain, which forms a 4-helix dimer that covers the opposite phenylalanine residue of the hairpin dimer. Collectively, the data provide the first structural model for CM1 autoinhibition and reveal evolutionary differences across species.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Abir Elfarkouchi"
    },
    {
      "name": "Arthur Vitard"
    },
    {
      "name": "Léa Mammri"
    },
    {
      "name": "Sébastien Brier"
    },
    {
      "name": "Lola Tanneur"
    },
    {
      "name": "Cécile Hu"
    },
    {
      "name": "Aurélien Thureau"
    },
    {
      "name": "Nicolas Joly"
    },
    {
      "name": "Françoise Ochsenbein"
    },
    {
      "name": "Paul T Conduit"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8ftxcyteyhks1.bib",
  "cited_by": 0,
  "doi": "10.1083/jcb.202605160",
  "fulltext_url": null,
  "id": "8ftxcyteyhks1",
  "issns": [
    "0021-9525",
    "1540-8140"
  ],
  "landing_url": "https://hal.science/hal-05756468v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
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      "hal",
      "2.sdv.bbm.bm"
    ],
    [
      "openalex",
      "1307|Microtubule and mitosis dynamics"
    ]
  ],
  "openalex": "W7215052183",
  "pdf_url": "https://hal.science/hal-05756468/document",
  "pmcid": null,
  "pmid": "42814412",
  "published": "2026-09-30",
  "publisher": "Rockefeller University Press",
  "ris": "https://research.draconitic.com/paper/8ftxcyteyhks1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:36Z",
      "native_id": "hal-05756468",
      "source": "hal",
      "url": "https://hal.science/hal-05756468v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.bm",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "A structural model for the autoinhibition of the γ-TuRC-activating CM1 domain",
  "title_text": "A structural model for the autoinhibition of the γ-TuRC-activating CM1 domain",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:36Z",
  "url": "https://research.draconitic.com/paper/8ftxcyteyhks1",
  "venue": "Journal of Cell Biology",
  "versions": []
}