{
  "abstract": "Pseudomonas aeruginosa is a pathogen responsible for healthcare-associated infections, and the emergence of carbapenem-resistant strains (CRPA) poses significant clinical challenges. Phage therapy is increasingly recognized as a promising strategy against drug-resistant bacteria. This study aimed to identify a phage capable of effectively lysing CRPA. Using CRPA clinical isolates (PA19N) as hosts, phages were isolated and purified from water samples via the double-layer agar plate assay. A P. aeruginosa phage, designated Henuyfy19N, was successfully isolated. Transmission electron microscopy revealed that it possesses a head with diameter of approximately 87.94 ± 1.32 nm and a tail length of about 130.25 ± 3.65 nm, and it forms plaques with clear, distinct margins. The phage exhibited an optimal multiplicity of infection (MOI) of 0.1 against PA19N, with a latent period of 10 min, and a rise period of 30 min. It demonstrated strong stability under various temperature and pH conditions but was sensitive to UV irradiation. Whole-genome sequencing showed that the phage has a 93,413 bp linear dsDNA genome with a G + C content of 49.42%, containing 161 ORFs and 16 tRNAs; no known virulence or antibiotic resistance genes were detected. Functional annotation predicted genes involved in structural proteins, DNA replication/packaging, and lysis. In vitro, Henuyfy19N effectively inhibited PA19N growth, reduced viable bacterial counts, and exhibited anti-biofilm activity by both disrupting and preventing biofilm formation. In Galleria mellonella larvae and a mouse infection model, the phage demonstrated therapeutic efficacy against PA19N infections. These findings establish Henuyfy19N as a safe, stable, and effective candidate for the treatment of CRPA infection.",
  "abstract_shown": true,
  "affiliations": [
    "Henan Province Engineering Technology Research Center of Rapid-Accuracy Medical Diagnostics, Department of Clinical Laboratory, The First Affiliated Hospital of Henan University, Henan University, Kaifeng, 475000, China.",
    "Kaifeng Engineering Technology Research Center for In Vitro Diagnostics, The First Affiliated Hospital of Henan University, Henan University, Kaifeng, 475000, China.",
    "Henan Province Engineering Technology Research Center of Rapid-Accuracy Medical Diagnostics, Department of Clinical Laboratory, The First Affiliated Hospital of Henan University, Henan University, Kaifeng, 475000, China. liqiming82@126.com.",
    "Kaifeng Engineering Technology Research Center for In Vitro Diagnostics, The First Affiliated Hospital of Henan University, Henan University, Kaifeng, 475000, China. liqiming82@126.com.",
    "State Key Laboratory of Antiviral Drugs, Henan University, Kaifeng, 475004, China. liqiming82@126.com."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Xin Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xinwei Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Dong Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiaqi He"
    },
    {
      "affs": [
        0
      ],
      "name": "Yingge Mao"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiaolian Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhigang Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaoquan Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Fang Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Li Wang"
    },
    {
      "affs": [
        2,
        3,
        4
      ],
      "name": "Qiming Li"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8h5n4a5aayzfs.bib",
  "cited_by": 0,
  "doi": "10.1186/s13568-026-02099-1",
  "fulltext_url": "https://europepmc.org/articles/PMC13728087",
  "id": "8h5n4a5aayzfs",
  "issns": [
    "2191-0855"
  ],
  "landing_url": "https://europepmc.org/article/MED/42972195",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7172528112",
  "pdf_url": "https://europepmc.org/articles/PMC13728087?pdf=render",
  "pmcid": "PMC13728087",
  "pmid": "42972195",
  "published": "2026-08-05",
  "publisher": "Springer",
  "ris": "https://research.draconitic.com/paper/8h5n4a5aayzfs.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:49Z",
      "native_id": "MED:42972195",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42972195"
    },
    {
      "first_seen": "2026-11-06T12:23:50Z",
      "native_id": "PMC13728087",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s13568-026-02099-1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2303|Bacteriophages and microbial interactions",
      "subfield": "ecology"
    }
  ],
  "title": "Characterization of phage Henuyfy19N against Pseudomonas aeruginosa and its efficacy in animal models.",
  "title_text": "Characterization of phage Henuyfy19N against Pseudomonas aeruginosa and its efficacy in animal models.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:09Z",
  "url": "https://research.draconitic.com/paper/8h5n4a5aayzfs",
  "venue": "AMB Express",
  "versions": []
}