{
  "abstract": "Replication fork reversal is a central response to replication stress, yet how a SNF2 translocase coordinates the distinct mechanical steps required for this reaction remains elusive. Here, we define the mechanism by which ZRANB3 catalyzes fork reversal using biochemical, biophysical, and single-molecule approaches. ZRANB3 binds fork DNA as a monomer at the junction, establishing the minimal functional unit required for activity. Mechanistically, ZRANB3 combines ATP-dependent localized duplex opening with ATP-independent strand annealing to facilitate four-way junction formation, followed by directional branch migration that supports long-range fork reversal. Single-molecule fluorescence analyses further demonstrate that monomeric ZRANB3 is sufficient to drive fork reversal. Together, these findings define how ZRANB3 integrates distinct DNA remodeling activities within a single enzyme to catalyze fork reversal.",
  "abstract_shown": true,
  "affiliations": [
    "Institute of Biochemical Sciences, National Taiwan University , Taipei 106 ,",
    "Department of Chemistry, National Taiwan University , Taipei 106 ,",
    "Institute of Biological Chemistry , Academia Sinica, Taipei 106 ,",
    "Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica , Taipei 106 ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yen-Ju Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Yu-Hui Chiu",
      "orcid": "0009-0004-5458-1119"
    },
    {
      "affs": [
        1
      ],
      "name": "Chieh-Kai Liu"
    },
    {
      "affs": [
        1
      ],
      "name": "Hsuan Hsu"
    },
    {
      "affs": [
        1
      ],
      "name": "Kuan-Chi Wang"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Guan-Ting Lian"
    },
    {
      "affs": [
        2
      ],
      "name": "Yi-An Chen"
    },
    {
      "affs": [
        2
      ],
      "name": "Sheng-Wei Lin"
    },
    {
      "affs": [
        2
      ],
      "name": "Hsin-Yung Yen"
    },
    {
      "affs": [
        1
      ],
      "name": "Hung-Wen Li",
      "orcid": "0000-0002-5614-3727"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Peter Chi",
      "orcid": "0000-0001-9229-8729"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8hdh191ykq888.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag1034",
  "fulltext_url": "https://europepmc.org/articles/PMC13728640",
  "id": "8hdh191ykq888",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1034/9010722",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "AcademicSubjects/SCI00010"
    ],
    [
      "heading",
      "Genome Integrity, Repair and Replication"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://academic.oup.com/nar/article-pdf/54/20/gkag1034/72841834/gkag1034.pdf",
  "pmcid": "PMC13728640",
  "pmid": "42974765",
  "published": "2026-10-01",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/8hdh191ykq888.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:21:05Z",
      "native_id": "10.1093/nar/gkag1034",
      "source": "crossref",
      "url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1034/9010722"
    },
    {
      "first_seen": "2026-11-04T15:20:49Z",
      "native_id": "MED:42974765",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42974765"
    },
    {
      "first_seen": "2026-11-07T12:21:35Z",
      "native_id": "PMC13728640",
      "source": "pmc",
      "url": "https://doi.org/10.1093/nar/gkag1034"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:0305-1048",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Monomeric ZRANB3 drives replication fork reversal",
  "title_text": "Monomeric ZRANB3 drives replication fork reversal",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:35Z",
  "url": "https://research.draconitic.com/paper/8hdh191ykq888",
  "venue": "Nucleic Acids Research",
  "versions": []
}