{
  "abstract": "Liposarcoma (LPS) is a common soft tissue sarcoma; however, its molecular pathogenesis and immune cell infiltration remain poorly understood. This study investigated potential driver genes and pathways in LPS and characterized immune cell infiltration patterns to identify potential markers for targeted therapy. Differentially expressed genes (DEGs) in LPS were analyzed by GO and KEGG pathway enrichment analysis. A protein-protein interaction network was constructed using the STRING database and visualized with Cytoscape. mRNA expression of genes with high |logFC| values was verified by RT-qPCR. Immune cell subsets were quantified using CIBERSORT. GO and KEGG analysis revealed significant functional clusters and pathways, and most verified genes were consistent with the bioinformatics analysis. Survival analysis showed that high expression of TYMS, KIF20A, BUB1B, LMNB1, RRM2, ZWINT, and RACGAP1 was significantly associated with poor overall survival and poor disease-free survival (DFS). High levels of TMSB15A, TPX2, PKM2, and PTTG1 were significantly associated with poor DFS alone. CIBERSORT analysis identified a significantly higher fraction of resting mast cells (MCs) in LPS tissues compared to normal fatty tissues (P < 0.05). TOP2A, IL-6, PCNA, CDK1, JUN, MYC, CCNB1, EGFR, ACACB, and BIRC5 were identified as potential diagnostic biomarkers of LPS, providing strong evidence for hub gene studies. Immune cell infiltration analysis further suggested that resting MCs may play a potential role in LPS development, although further experimental validation is warranted. Collectively, these findings clarify the molecular basis of LPS and provide a foundation for future research into its treatment.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China",
    "Department of Critical Care Unit, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China"
  ],
  "article_type": "Research Article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Chunxiao Liu"
    },
    {
      "affs": [
        1
      ],
      "name": "Yanhua Wang"
    },
    {
      "name": "Qiuxia Liu"
    },
    {
      "name": "Sha Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8j8ydewqrs4zw.bib",
  "cited_by": 0,
  "doi": "10.1371/journal.pone.0358180",
  "fulltext_url": null,
  "id": "8j8ydewqrs4zw",
  "issns": [
    "1932-6203"
  ],
  "landing_url": "https://doi.org/10.1371/journal.pone.0358180",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2740|Sarcoma Diagnosis and Treatment"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cell processes/Cell cycle and cell division"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular structures and organelles"
    ],
    [
      "plos",
      "/Biology and life sciences/Cell biology/Cellular types/Animal cells/Immune cells"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene expression/Gene regulation"
    ],
    [
      "plos",
      "/Biology and life sciences/Genetics/Gene identification and analysis/Genetic networks"
    ],
    [
      "plos",
      "/Biology and life sciences/Immunology/Immune cells"
    ],
    [
      "plos",
      "/Computer and information sciences/Network analysis/Genetic networks"
    ],
    [
      "plos",
      "/Medicine and health sciences/Immunology/Immune cells"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Sarcoma"
    ],
    [
      "plos",
      "/Medicine and health sciences/Oncology/Cancers and neoplasms/Sarcoma/Liposarcoma"
    ]
  ],
  "openalex": "W7212294742",
  "pdf_url": "https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0358180&type=printable",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-11",
  "publisher": "Public Library of Science",
  "ris": "https://research.draconitic.com/paper/8j8ydewqrs4zw.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:56Z",
      "native_id": "10.1371/journal.pone.0358180",
      "source": "plos",
      "url": "https://doi.org/10.1371/journal.pone.0358180"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "plos:gene expression",
      "subfield": "molecular-biology"
    },
    {
      "field": "biology",
      "origin": "plos:cell biology",
      "subfield": "cell-biology"
    },
    {
      "field": "biology",
      "origin": "plos:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Identification of functional genes and immune cell infiltration analysis in liposarcoma",
  "title_text": "Identification of functional genes and immune cell infiltration analysis in liposarcoma",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:56Z",
  "url": "https://research.draconitic.com/paper/8j8ydewqrs4zw",
  "venue": "PLOS One",
  "versions": []
}