{
  "abstract": "To investigate the regulatory function of the tomato NAC transcription factor SlNAC2-like under high-temperature stress, the high-temperature-tolerant line Hm 2-2 and the high-temperature-sensitive line BY 1-2 were used as experimental materials. The SlNAC2-like gene was cloned from tomato leaves via reverse transcription PCR (RT-PCR), and its encoded protein was characterized using bioinformatics tools, phylogenetic analysis, and expression profiling. Subcellular localization and yeast self-activation assays were performed to determine protein localization and transcriptional activity. Virus-induced gene silencing (VIGS) was employed to generate SlNAC2-like-silenced plants, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as proline (Pro) and malondialdehyde (MDA) contents, were measured under high-temperature stress using spectrophotometry. The results showed that the SlNAC2-like cDNA fragment was 1044 bp in length, with an 876 bp open reading frame encoding 291 amino acids containing a highly conserved NAM domain. The protein was localized in the nucleus and exhibited transcriptional activation activity. Quantitative real-time PCR (qRT-PCR) analysis revealed that SlNAC2-like expression was tissue-specific and induced by high temperature (42 °C), abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA). Silencing of SlNAC2-like compromised tomato high-temperature tolerance, as evidenced by reduced SOD, POD, and CAT activities, decreased Pro content, and a marked increase in MDA content, along with significant downregulation of genes involved in ABA, SA, and jasmonic acid (JA) signaling pathways. Collectively, these findings suggest that SlNAC2-like acts as a positive regulator of tomato tolerance to high-temperature stress and that its function is associated with antioxidant defense and the ABA, SA, and JA signaling pathways. However, the present study does not establish how SlNAC2-like regulates ROS homeostasis or hormone signaling, and identification of its direct target genes and interacting partners requires further experimental validation. This study provides valuable genetic resources and a theoretical basis for molecular breeding of stress-resistant tomato varieties.",
  "abstract_shown": true,
  "affiliations": [
    "School of Life Sciences and Environmental Resources, Yichun University, Yichun 336000, China.",
    "Key Laboratory of Germplasm Innovation and Green High-Value Utilization for Specialty Crops in Western Jiangxi, Jiangxi Education Department, Yichun University, Yichun 336000, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Lingzeng Lv"
    },
    {
      "affs": [
        0
      ],
      "name": "Lian Duan"
    },
    {
      "affs": [
        0
      ],
      "name": "Jingwei Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Haibo Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qin Shao"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Na Chen"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8jf6ea9t97b96.bib",
  "cited_by": 0,
  "doi": "10.3390/plants15203213",
  "fulltext_url": "https://europepmc.org/articles/PMC13709242",
  "id": "8jf6ea9t97b96",
  "issns": [
    "2223-7747"
  ],
  "landing_url": "https://europepmc.org/article/MED/42943166",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Stress Responses and Tolerance"
    ]
  ],
  "openalex": "W7223002264",
  "pdf_url": "https://europepmc.org/articles/PMC13709242?pdf=render",
  "pmcid": "PMC13709242",
  "pmid": "42943166",
  "published": "2026-10-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8jf6ea9t97b96.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42943166",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42943166"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Stress Responses and Tolerance",
      "subfield": "plant-biology"
    }
  ],
  "title": "Cloning, Expression Analysis, and Functional Characterization of the NAC Transcription Factor SlNAC2-like from Tomato.",
  "title_text": "Cloning, Expression Analysis, and Functional Characterization of the NAC Transcription Factor SlNAC2-like from Tomato.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/8jf6ea9t97b96",
  "venue": "Plants (Basel, Switzerland)",
  "versions": []
}