{
  "abstract": "Background: Preoperative fine-needle aspiration (FNA) cytology and conventional genetic testing often fail on indeterminate thyroid nodules and malignancies lacking typical driver mutations. We validated two candidate lncRNAs, lnc-ABCA12-5:2 and lnc-MPEG1-1:1, initially screened in papillary thyroid carcinoma (PTC) tissues, using preoperative FNA and postoperative formalin-fixed paraffin-embedded (FFPE) specimens. Methods: Diagnostic performance was assessed in two independent cohorts encompassing diverse Bethesda categories and thyroid carcinoma subtypes. Molecular correlations and clinicopathological associations were also examined. Results: Both lncRNAs exhibited excellent diagnostic efficacy for PTC in preoperative FNA samples (area under the curve values: 0.923 and 0.968; sensitivity > 0.85; specificity > 0.90). lnc-ABCA12-5:2 achieved 100% specificity and 100% positive predictive value in Bethesda III–IV nodules, and was an independent predictor alongside BRAF V600E. Integrating lnc-ABCA12-5:2 with BRAF V600E testing raised diagnostic accuracy in indeterminate nodules from 89.5% to 95.0% (95.7% sensitivity, 92.9% specificity). lnc-ABCA12-5:2 positivity increased progressively with higher Bethesda grades (p-trend < 0.001), achieved 100% positivity in high-grade aggressive carcinomas, and remained elevated (83.6%) in genetically wild-type malignant nodules. Its expression strongly correlated with aggressive molecular signatures (including TERT, TP53, PI3K/AKT/PTEN alterations), yielding 100% positivity in such cases. In vitro cellular functional assays further confirmed that lnc-ABCA12-5:2 overexpression significantly promoted cell proliferation, colony formation, and migration in PTC cell lines. Conclusions: lnc-ABCA12-5:2 and lnc-MPEG1-1:1 are robust preoperative biomarkers for PTC. lnc-ABCA12-5:2 fills critical diagnostic gaps in indeterminate and genetically wild-type thyroid malignancies, and correlates with aggressive tumor molecular profiles, underscoring its translational potential for clinical risk stratification.",
  "abstract_shown": true,
  "affiliations": [
    "State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, China",
    "Department of Head and Neck, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, China",
    "Department of Clinical Laboratory, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, China",
    "Department of Pathology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China",
    "Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China",
    "Department of Molecular Diagnostics, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xuan Su"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Wen-na Xu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Bu Zou"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Chan Huang"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Cai-yun He"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Zu-lu Ye"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8kmemja9rk18g.bib",
  "cited_by": 0,
  "doi": "10.32604/or.2026.088872",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13732376/",
  "id": "8kmemja9rk18g",
  "issns": [
    "0965-0407",
    "1555-3906"
  ],
  "landing_url": "https://doi.org/10.32604/or.2026.088872",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2712|Thyroid Cancer Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7222960178",
  "pdf_url": "https://www.techscience.com/files/onlinefirst/2026/10.19/TSP_OR_88872/TSP_OR_88872.pdf",
  "pmcid": "PMC13732376",
  "pmid": "42985401",
  "published": "2026-10-28",
  "publisher": "Tech Science Press",
  "ris": "https://research.draconitic.com/paper/8kmemja9rk18g.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:22:22Z",
      "native_id": "PMC13732376",
      "source": "pmc",
      "url": "https://doi.org/10.32604/or.2026.088872"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2712|Thyroid Cancer Diagnosis and Treatment",
      "subfield": "endocrinology-metabolism"
    }
  ],
  "title": "A Key Biomarker lnc-ABCA12-5:2 Compensating Diagnostic Deficiencies for Indeterminate Thyroid Nodules and Genetically Wild-Type Thyroid Malignancies",
  "title_text": "A Key Biomarker lnc-ABCA12-5:2 Compensating Diagnostic Deficiencies for Indeterminate Thyroid Nodules and Genetically Wild-Type Thyroid Malignancies",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:22:22Z",
  "url": "https://research.draconitic.com/paper/8kmemja9rk18g",
  "venue": "Oncology Research",
  "versions": []
}