{
  "abstract": "Ferroptosis is an iron-dependent regulated cell death characterized by iron accumulation and lipid peroxidation. The liver represents the primary site of iron-overload injury due to its role in iron metabolism, but the potential role of ferroptosis in the biliary epithelium is yet unknown. The study investigated the recruitment of ferroptosis under control conditions and after bile duct ligation (BDL), highlighting the effects of ferroptosis inducers and inhibitors in a cell line of cholangiocytes. In addition, we identified iron deposits through Perls Prussian blue as well as the presence of proteins such as ferritin, transferrin receptor 1 (CD71), glutathione peroxidase-4 (GPX4) and acyl-CoA synthetase long-chain family member 4 (ACSL4). The results showed an increase in the expression of ferritin, CD71 and ACSL4 in the BDL model, whereas GPX4 was reduced in the cholestatic model in comparison to the control. In addition, sorafenib failed to trigger ferroptosis in cholangiocytes, whereas erastin was a potent inducer. Conversely, ferrostatin-1 (Fer-1) did not inhibit ferroptosis, while β-mercaptoethanol (βME) could protect cholangiocytes from ferroptosis-induced cell death. All these findings are important for understanding the involvement of ferroptosis in cholangiocyte pathophysiology and may support additional studies regarding the possible application of ferroptosis modulators in the treatment of liver fibrosis.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome",
    "Interdisciplinary Department of Well-Being, Health and Environment Sustainability (BESSA), Sapienza University of Rome, Rieti",
    "Department of Physiology and Pharmacology ‘V. Erspamer’, Sapienza University of Rome",
    "Department of Experimental Morphology and Microscopy, Integrated Research Center (PRAAB), Campus Biomedico University of Rome",
    "Department of Life Sciences, Health and Health Professions, LINK Campus University, Rome",
    "Division of Health Sciences, Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Italy"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Romina Mancinelli"
    },
    {
      "affs": [
        0
      ],
      "name": "Arianna Casini"
    },
    {
      "affs": [
        0
      ],
      "name": "Stefano Leone"
    },
    {
      "affs": [
        1
      ],
      "name": "Ludovica Ceci"
    },
    {
      "affs": [
        0
      ],
      "name": "Rosa Vaccaro"
    },
    {
      "affs": [
        2
      ],
      "name": "Antonella Di Sotto"
    },
    {
      "affs": [
        3
      ],
      "name": "Giorgio Vivacqua"
    },
    {
      "affs": [
        0
      ],
      "name": "Rosemarie Heyn"
    },
    {
      "affs": [
        0
      ],
      "name": "Ludovica Garro"
    },
    {
      "affs": [
        0
      ],
      "name": "Marco Tagliafierro"
    },
    {
      "affs": [
        0
      ],
      "name": "Filippo Maria Bassi"
    },
    {
      "affs": [
        0
      ],
      "name": "Emanuele Bocci"
    },
    {
      "affs": [
        4
      ],
      "name": "Andrea Bassi"
    },
    {
      "affs": [
        0
      ],
      "name": "Luigi Pannarale"
    },
    {
      "affs": [
        5
      ],
      "name": "Antonio Franchitto"
    },
    {
      "affs": [
        0
      ],
      "name": "Paolo Onori"
    },
    {
      "affs": [
        0
      ],
      "name": "Eugenio Gaudio"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8n0k8eeeme4ww.bib",
  "cited_by": 0,
  "doi": "10.4081/ejh.2026.4615",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13735480/",
  "id": "8n0k8eeeme4ww",
  "issns": [
    "1121-760X",
    "2038-8306"
  ],
  "landing_url": "https://doi.org/10.4081/ejh.2026.4615",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1306|Ferroptosis and cancer prognosis"
    ]
  ],
  "openalex": "W7224131807",
  "pdf_url": "https://www.ejh.it/ejh/article/download/4615/3835",
  "pmcid": "PMC13735480",
  "pmid": "42949000",
  "published": "2026-10-28",
  "publisher": "PAGEPress",
  "ris": "https://research.draconitic.com/paper/8n0k8eeeme4ww.ris",
  "sources": [
    {
      "first_seen": "2026-11-09T12:21:09Z",
      "native_id": "PMC13735480",
      "source": "pmc",
      "url": "https://doi.org/10.4081/ejh.2026.4615"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|Ferroptosis and cancer prognosis",
      "subfield": "oncology"
    }
  ],
  "title": "Discovering ferroptosis in biliary epithelium during liver fibrosis",
  "title_text": "Discovering ferroptosis in biliary epithelium during liver fibrosis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:09Z",
  "url": "https://research.draconitic.com/paper/8n0k8eeeme4ww",
  "venue": "European Journal of Histochemistry : EJH",
  "versions": []
}