{
  "abstract": "Broccoli (Brassica oleracea var. italica) is a widely cultivated cruciferous vegetable recognized for its rich content of bioactive phytochemicals with documented health-promoting properties. Fresh broccoli florets represent a rich yet underutilized source of bioactive phytochemicals, particularly glucosinolates, with established health benefits. This study optimized ultrasonic-assisted extraction (UAE) using aqueous ethanol to recover glucosinolates and sulforaphane. Total glucosinolate content (TGC) was quantified spectrophotometrically, while sulforaphane, glucoraphanin and glucobrassicin were determined by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS). Under the optimized UAE conditions (25% ethanol, 41 °C, 32 min), the predicted yield of TGC was 46.6 mg of sinigrin equivalents (SE)/g dry weight (DW), while the actual yield achieved was 36.6 ± 4.4 mg of SE/g DW. Among the compared extraction methods, UAE with 25% ethanol yielded the highest TGC, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) with values of 35.5 ± 1.26 mg of SE/g DW, 21.2 ± 2.6 µmol of Trolox equivalents (TE)/g DW, and 156 ± 15.2 µmol of TE/g DW, respectively, with TGC and FRAP being significantly higher than all other methods (p < 0.05), while ORAC differences were not statistically significant. In vitro anti-elastase activity was the greatest in the extracts prepared using shaker-assisted extraction (IC₅₀ of 0.51 mg/mL) and UAE-25% ethanol (IC₅₀ of 0.86 mg/mL). Collectively, these findings position UAE with aqueous ethanol as a promising extraction approach for recovering glucosinolates and sulforaphane from broccoli florets for cosmeceutical applications, providing a basis for future scale-up and process optimization studies.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Chandrika Chaturvedi",
      "orcid": "0009-0002-4673-2453"
    },
    {
      "affs": [
        0
      ],
      "name": "H P Vasantha Rupasinghe",
      "orcid": "0000-0003-3435-0052"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8qk88adc7579q.bib",
  "cited_by": 0,
  "doi": "10.3390/foods15193431",
  "fulltext_url": "https://europepmc.org/articles/PMC13664207",
  "id": "8qk88adc7579q",
  "issns": [
    "2304-8158"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883538",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Genomics, phytochemicals, and oxidative stress"
    ]
  ],
  "openalex": "W7214281335",
  "pdf_url": "https://europepmc.org/articles/PMC13664207?pdf=render",
  "pmcid": "PMC13664207",
  "pmid": "42883538",
  "published": "2026-09-25",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8qk88adc7579q.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883538",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883538"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Genomics, phytochemicals, and oxidative stress",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Glucosinolate- and Sulforaphane-Enriched Broccoli Extract as a Potential Dual-Action Antioxidant and Elastase Inhibitor.",
  "title_text": "Glucosinolate- and Sulforaphane-Enriched Broccoli Extract as a Potential Dual-Action Antioxidant and Elastase Inhibitor.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/8qk88adc7579q",
  "venue": "Foods (Basel, Switzerland)",
  "versions": []
}