{
  "abstract": "Introduction\n\nTumor-associated microbiota have been implicated in cancer biology, but the relationship between bacteria detected on the biopsy surface and those detected within tumor tissue remains poorly defined.Methods\n\nIn this study, we profiled paired biopsy-surface swabs and tissue biopsies from patients with nasopharyngeal carcinoma (NPC) and benign nasopharyngeal lymphoid hyperplasia (NLH) controls using five-region 16S rRNA gene sequencing. Matched bulk RNA-seq data were analyzed to explore associations between microbial features, Epstein-Barr virus (EBV) transcription, host pathway activity, and inferred immune-cell composition.Results\n\nTissue-associated microbial profiles showed stronger separation between NPC and NLH than biopsy-surface profiles in this discovery cohort. Several NPC-enriched species-level taxonomic assignments were associated with higher EBV transcript levels, proliferative and cell-cycle pathway activity, and lower inferred CD8 T-cell and plasmacytoid dendritic-cell signatures.Discussion\n\nThese findings indicate that NPC tissue contains microbial signatures that differ from biopsy-surface communities and support further validation of tissue-associated microbe-EBV-host relationships.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.",
    "Department of Head and Neck Tumor Surgery, Guangxi Medical University Cancer Hospital, Nanning, China.",
    "Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumours (Guangxi Medical University), Ministry of Education, Nanning, China.",
    "Department of Otorhinolaryngology Head and Neck Surgery, Binzhou Central Hospital, Binzhou, Shandong, China.",
    "School of Life Sciences Institute, Guangxi Medical University, Nanning, China.",
    "School of International Education, Guangxi Medical University, Nanning, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Nan Shi"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Zongjian Huang"
    },
    {
      "affs": [
        0,
        2,
        3
      ],
      "name": "Xuesong Wang"
    },
    {
      "affs": [
        1,
        2,
        4,
        5
      ],
      "name": "Ying Xie"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Anzhou Tang"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Wei Xia"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8rdjw4ths9vd4.bib",
  "cited_by": 0,
  "doi": "10.3389/fcimb.2026.1892233",
  "fulltext_url": "https://europepmc.org/articles/PMC13713345",
  "id": "8rdjw4ths9vd4",
  "issns": [
    "2235-2988"
  ],
  "landing_url": "https://europepmc.org/article/MED/42952527",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2733|Head and Neck Cancer Studies"
    ]
  ],
  "openalex": "W7221754045",
  "pdf_url": "https://europepmc.org/articles/PMC13713345?pdf=render",
  "pmcid": "PMC13713345",
  "pmid": "42952527",
  "published": "2026-10-14",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/8rdjw4ths9vd4.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:34Z",
      "native_id": "MED:42952527",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42952527"
    },
    {
      "first_seen": "2026-10-31T12:23:54Z",
      "native_id": "PMC13713345",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fcimb.2026.1892233"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2733|Head and Neck Cancer Studies",
      "subfield": "general"
    }
  ],
  "title": "Paired biopsy-surface and tissue microbiome profiling reveals tissue-associated bacterial signatures in nasopharyngeal carcinoma.",
  "title_text": "Paired biopsy-surface and tissue microbiome profiling reveals tissue-associated bacterial signatures in nasopharyngeal carcinoma.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-01T12:21:02Z",
  "url": "https://research.draconitic.com/paper/8rdjw4ths9vd4",
  "venue": "Frontiers in cellular and infection microbiology",
  "versions": []
}