{
  "abstract": "Background\n\nIntegrative transcriptomic analyses can prioritize genes associated with chronic obstructive pulmonary disease (COPD), but expression associations require experimental assessment.Methods\n\nSingle-cell GSE173896 and bulk GSE57148 data were analyzed using clustering, co-expression networks, CellChat, differential expression, immune deconvolution, pseudotime, and virtual knockout analysis. A five-gene logistic nomogram was developed in GSE57148. Clinical RT-qPCR included 30 participants with COPD and 30 without COPD. Two independent IL6-targeting siRNAs were examined in HBEC3-KT and BEAS-2B cells using epithelial-macrophage co-culture, cytokine measurements, and wound-healing assays.Results\n\nNHSL2, CDKL5, CRIP1, TNNI2, and IL6 were prioritized as candidate genes. Clinical RT-qPCR supported higher CDKL5, IL6, and NHSL2 expression and lower CRIP1 and TNNI2 expression in COPD. IL6 knockdown reduced epithelial IL6 expression and attenuated several macrophage-associated transcriptional responses in co-culture after epithelial cigarette smoke extract exposure. Secretory responses varied by cell line and siRNA; co-culture IL-10 reductions were significant in the HBEC3-KT system but not in the BEAS-2B system. IL6 knockdown also reduced epithelial wound closure. The nomogram was evaluated within its development cohort and lacked independent external validation.Conclusion\n\nThe study identifies five COPD-associated candidate genes and provides experimental evidence linking epithelial IL6 perturbation to selected co-culture responses and wound closure. These findings do not establish a COPD-specific mechanism or therapeutic benefit. The nomogram remains exploratory and requires independent validation.",
  "abstract_shown": true,
  "affiliations": [
    "Graduate School, Tianjin Medical University, Tianjin, People's Republic of China.",
    "Thoracic Surgery Clinical College, Tianjin Medical University, Tianjin, People's Republic of China.",
    "Graduate School, Tianjin University, Tianjin, People's Republic of China.",
    "Department of Thoracic Surgery, Tianjin Chest Hospital, Tianjin, People's Republic of China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Jianyu Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Han Zhang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qiuqiao Mu"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Xin Li"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Daqiang Sun",
      "orcid": "0000-0003-2222-4657"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8rvh84twnqx98.bib",
  "cited_by": 0,
  "doi": "10.2147/copd.s629378",
  "fulltext_url": "https://europepmc.org/articles/PMC13726642",
  "id": "8rvh84twnqx98",
  "issns": [
    "1176-9106",
    "1178-2005"
  ],
  "landing_url": "https://europepmc.org/article/MED/42974151",
  "language": "eng",
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2740|Chronic Obstructive Pulmonary Disease (COPD) Research"
    ]
  ],
  "openalex": "W7224536651",
  "pdf_url": "https://europepmc.org/articles/PMC13726642?pdf=render",
  "pmcid": "PMC13726642",
  "pmid": "42974151",
  "published": "2026-10-29",
  "publisher": "Dove Press",
  "ris": "https://research.draconitic.com/paper/8rvh84twnqx98.ris",
  "sources": [
    {
      "first_seen": "2026-11-04T15:20:47Z",
      "native_id": "MED:42974151",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42974151"
    },
    {
      "first_seen": "2026-11-05T12:22:56Z",
      "native_id": "PMC13726642",
      "source": "pmc",
      "url": "https://doi.org/10.2147/copd.s629378"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2740|Chronic Obstructive Pulmonary Disease (COPD) Research",
      "subfield": "general"
    }
  ],
  "title": "Identification of Five Candidate Genes and Development of an Exploratory Diagnostic Nomogram for COPD Using Integrative Single-Cell and Bulk Transcriptomic Analyses.",
  "title_text": "Identification of Five Candidate Genes and Development of an Exploratory Diagnostic Nomogram for COPD Using Integrative Single-Cell and Bulk Transcriptomic Analyses.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:22:32Z",
  "url": "https://research.draconitic.com/paper/8rvh84twnqx98",
  "venue": "International journal of chronic obstructive pulmonary disease",
  "versions": []
}