{
  "abstract": "The ARP2/3 complex generates branched actin networks that regulate membrane dynamics across eukaryotes. In plants, ARP2/3 is activated primarily by the WAVE/SCAR complex and is essential for cell morphogenesis, yet its spatiotemporal behavior in living cells remains poorly understood. Using high-resolution live-cell microscopy, we found that, in addition to their previously reported stable accumulation at three-way cell junctions and peroxisomes, WAVE/SCAR and ARP2/3 subunits also form abundant, highly dynamic, short-lived assemblies in the cortical cytoplasm, with lifetimes of only a few seconds. Genetic and colocalization analyses further revealed that only a minority of observed complexes are fully assembled and active, indicating that plant cells maintain a large pool of partially assembled or inactive ARP2/3 structures. Our data indicate that microtubules influence the abundance of cortical assemblies, whereas actin primarily affects their dynamics. Importantly, our analysis demonstrated a spatial and functional association between dynamic ARP2/3 foci and exocytotic events at the plasma membrane. Together, our findings suggest that the cytoplasm of plant epidermal cells contains a large reservoir of ARP2/3 complexes whose localized activation is tightly regulated and associated with membrane trafficking.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic."
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "B. Jelinkova"
    },
    {
      "name": "M. Voloshina"
    },
    {
      "name": "K. Liebezeit"
    },
    {
      "name": "J. Krtkova"
    },
    {
      "name": "J. Garcia-Gonzalez"
    },
    {
      "name": "S. Vosolsobe"
    },
    {
      "name": "A. Harmanec"
    },
    {
      "name": "K. Malinska"
    },
    {
      "name": "A. Stelate"
    },
    {
      "name": "E. Kollarova"
    },
    {
      "name": "A. Baquero Forero"
    },
    {
      "name": "V. Zarsky"
    },
    {
      "affs": [
        0
      ],
      "name": "K. Schwarzerova"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8th697gyyfhk7.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.07.10.737699",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.07.10.737699v2.full",
  "id": "8th697gyyfhk7",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.07.10.737699v2",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "plant biology"
    ],
    [
      "openalex",
      "1307|Cellular transport and secretion"
    ]
  ],
  "openalex": "W7167900543",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.07.10.737699v2.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-10",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8th697gyyfhk7.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:52Z",
      "native_id": "10.64898/2026.07.10.737699",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.07.10.737699v2"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:plant biology",
      "subfield": "plant-biology"
    }
  ],
  "title": "In vivo imaging uncovers an abundant but rarely active pool of plant ARP2/3 and its link to exocytosis and endocytosis",
  "title_text": "In vivo imaging uncovers an abundant but rarely active pool of plant ARP2/3 and its link to exocytosis and endocytosis",
  "type": "preprint",
  "updated_at": "2026-10-30T12:22:52Z",
  "url": "https://research.draconitic.com/paper/8th697gyyfhk7",
  "venue": "bioRxiv",
  "versions": []
}