{
  "abstract": "Background:: Testicular torsion is a urological emergency that obstructs blood flow and causes ischemic damage, potentially leading to infertility or orchiectomy. This study aimed to evaluate the combined effects of mesenchymal stem cells (MSCs) and crocin on testicular ischemia-reperfusion (I/R) injury in rats.\n\nMaterials and Methods:: In this experimental study, 50 adults male Wistar rats were randomly divided into five groups: sham-operated (no ischemia or treatment), ischemia only, crocin-treated, MSC-treated, and combined crocin and MSCs. Testicular ischemia was induced by rotating the left testis for three hours, followed by reperfusion. Crocin (100 mg/kg) was administered intraperitoneally, and MSCs (3×10⁴ cells) were administered intraperitoneally. After 21 days of reperfusion, histological evaluation, using the Johnsen spermatogenesis scoring system, and assessment of oxidative stress markers [malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant status (TAS)] were performed.\n\nResults:: Combination therapy significantly improved the Johnsen score compared to the ischemia-only group (P<0.05) indicating enhanced spermatogenesis. MDA levels were significantly reduced, while antioxidant enzyme activities (SOD, GPx and TAS) were significantly increased in the combined treatment group compared to the untreated ischemic group (P<0.05). Each of crocin and MSCs individually exerted protective effects; however, the combined treatment showed a more pronounced overall improvement in testicular structure and oxidative status.\n\nConclusion:: Co-administration of MSCs and crocin effectively attenuated testicular I/R injury in rats by improving spermatogenesis and antioxidant defense, suggesting potential therapeutic value for reducing testicular torsion–related damage.",
  "abstract_shown": true,
  "affiliations": [
    "Faculty of Veterinary Medicine, SR.C., Islamic Azad University, Tehran, Iran",
    "Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, SR.C., Islamic Azad University, Tehran, Iran",
    "Applied Virology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran",
    "Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Saman Boostani"
    },
    {
      "affs": [
        1
      ],
      "name": "Ahmad Asghari"
    },
    {
      "affs": [
        2
      ],
      "name": "Hadi Esmaeili Gouvarchin Ghaleh"
    },
    {
      "affs": [
        3
      ],
      "name": "Parviz Tajik"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8y6vaf4ssr28f.bib",
  "cited_by": 0,
  "doi": "10.22074/ijfs.2026.2070505.1946",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13710916/",
  "id": "8y6vaf4ssr28f",
  "issns": [
    "2008-076X",
    "2008-0778"
  ],
  "landing_url": "https://doi.org/10.22074/ijfs.2026.2070505.1946",
  "language": "en",
  "licence": "CC-BY-NC-3.0",
  "licence_label": "CC BY-NC 3.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/3.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Andrology"
    ],
    [
      "heading",
      "Original Article"
    ],
    [
      "heading",
      "Stem Cells Biology"
    ],
    [
      "openalex",
      "2743|Sperm and Testicular Function"
    ]
  ],
  "openalex": "W7223320324",
  "pdf_url": null,
  "pmcid": "PMC13710916",
  "pmid": "42929013",
  "published": "2026-10-03",
  "publisher": "Royan Institute",
  "ris": "https://research.draconitic.com/paper/8y6vaf4ssr28f.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:47Z",
      "native_id": "PMC13710916",
      "source": "pmc",
      "url": "https://doi.org/10.22074/ijfs.2026.2070505.1946"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2743|Sperm and Testicular Function",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Role of Crocin and Mesenchymal Stem Cells Combined Therapy in Reducing Testicular Ischemia-Reperfusion Injury in Rats",
  "title_text": "Role of Crocin and Mesenchymal Stem Cells Combined Therapy in Reducing Testicular Ischemia-Reperfusion Injury in Rats",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:47Z",
  "url": "https://research.draconitic.com/paper/8y6vaf4ssr28f",
  "venue": "International Journal of Fertility & Sterility",
  "versions": []
}