{
  "abstract": "Bacteriophages (phages) infecting Gram-positive bacteria must bind to host receptors across thick cell walls to initiate infection, yet the underlying structural mechanisms remain unclear. Here, we report cryo-electron microscopy structures of the Oenococcus oeni siphophage OE33PA, providing the first high-resolution view of a phage infecting this wine-industry bacterium. While the overall virion architecture is conserved, the adhesion device displays distinctive features. Its receptor-binding proteins adopt multiple orientations, revealing an intrinsically dynamic assembly. In situ cryo-electron tomography captures distinct conformations upon host attachment, providing rare structural insight into interactions with Gram-positive hosts. Functional assays further show that a mobile carbohydrate-binding module in the distal tail protein mediates host cell binding. Additionnally, the tape measure protein adopts a hexameric organization, updating the prevailing trimeric model in siphophages. Together, these findings reveal a dynamic adhesion device in a phage infecting Gram-positive bacteria and highlight the structural and functional diversity of phages.",
  "abstract_shown": true,
  "affiliations": [
    "CNRS"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "name": "L. Schmitt"
    },
    {
      "name": "A. Chaib"
    },
    {
      "name": "D. Ptchelkine"
    },
    {
      "name": "E. Kandiah"
    },
    {
      "name": "C. Le Marrec"
    },
    {
      "name": "C. Cambillau"
    },
    {
      "affs": [
        0
      ],
      "name": "A. Goulet"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8ykb2yrjhmnyq.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.05.20.726473",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.05.20.726473v3.full",
  "id": "8ykb2yrjhmnyq",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.05.20.726473v3",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "openalex",
      "2303|Bacteriophages and microbial interactions"
    ]
  ],
  "openalex": "W7162094861",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.05.20.726473v3.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-05-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8ykb2yrjhmnyq.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T12:20:57Z",
      "native_id": "10.64898/2026.05.20.726473",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.05.20.726473v3"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Dynamic adhesion device of phage OE33PA drives Gram-positive host recognition",
  "title_text": "Dynamic adhesion device of phage OE33PA drives Gram-positive host recognition",
  "type": "preprint",
  "updated_at": "2026-11-04T00:21:01Z",
  "url": "https://research.draconitic.com/paper/8ykb2yrjhmnyq",
  "venue": "bioRxiv",
  "versions": []
}