{
  "abstract": "蛋白质糖基化和磷酸化均是生命体中重要的翻译后修饰，它们影响着蛋白质的空间构象、生物活性、运输和定位，在细胞生长和分裂、信号转导、代谢途径等特定生物过程中发挥着至关重要的作用。本研究利用尿素配体中的氢原子与1，3，5-三（4-苯硼酸）苯配体中硼酸根的氧原子形成氢键，制备了一种双配体氢键有机框架材料（Fe3O4@HOF），并用于复杂实际样品中糖基化肽（糖肽）与磷酸化肽（磷肽）的富集。基于该双配体磁性HOF材料，建立了磁性固相萃取-质谱分析（MSPE-MS）平台，以标准蛋白酶解物为模型，考察了该方法对糖肽和磷肽的富集能力，检出限分别低至0.4和0.1 fmol/μL。结果表明，该方法在人唾液和血清样本中低丰度糖肽和磷肽的分析中展现出较好的选择性及分离能力，为高通量糖基化及磷酸化蛋白质组学分析提供了新的思路。",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Yihan YANG"
    },
    {
      "name": "Chengyu CHENG"
    },
    {
      "name": "Yanjun LIU"
    },
    {
      "name": "Zhaoqing XIAO"
    },
    {
      "name": "Junwei YANG"
    },
    {
      "name": "Songrui LI"
    },
    {
      "name": "Haijiao ZHENG"
    },
    {
      "name": "Qiong JIA"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8z9bpckct5d72.bib",
  "cited_by": 0,
  "doi": "10.3724/sp.j.1123.2026.01031",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13729055/",
  "id": "8z9bpckct5d72",
  "issns": [
    "1000-8713",
    "1872-2059"
  ],
  "landing_url": "https://doi.org/10.3724/sp.j.1123.2026.01031",
  "language": "zh",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Articles"
    ],
    [
      "heading",
      "O658"
    ],
    [
      "heading",
      "a"
    ],
    [
      "openalex",
      "1607|Advanced Proteomics Techniques and Applications"
    ]
  ],
  "openalex": "W7225167310",
  "pdf_url": null,
  "pmcid": "PMC13729055",
  "pmid": "42973225",
  "published": "2026-11-07",
  "publisher": "Editorial Office of Chinese Journal of Chromatography",
  "ris": "https://research.draconitic.com/paper/8z9bpckct5d72.ris",
  "sources": [
    {
      "first_seen": "2026-11-07T12:21:53Z",
      "native_id": "PMC13729055",
      "source": "pmc",
      "url": "https://doi.org/10.3724/sp.j.1123.2026.01031"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1607|Advanced Proteomics Techniques and Applications",
      "subfield": "physical"
    }
  ],
  "title": "双配体磁性氢键有机框架材料的制备及用于糖基化肽与磷酸化肽的富集",
  "title_text": "双配体磁性氢键有机框架材料的制备及用于糖基化肽与磷酸化肽的富集",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:21:53Z",
  "url": "https://research.draconitic.com/paper/8z9bpckct5d72",
  "venue": "Chinese Journal of Chromatography",
  "versions": []
}