{
  "abstract": "Controlling protein localization through chemical biology tools is essential for mechanistic interrogations, functional perturbations, and therapeutic development. While existing tools cover a wide range of applications, no single approach simultaneously enables inducible trapping, controlled release, and visualization of a protein of interest (POI). To address this gap, we developed Biotin-Operated Ligand Trapping (BOLT), which combines a HaloTag-fused POI with a streptavidin trap. Desthiobiotin-conjugated Janelia Fluor HaloTag ligands enable POI visualization, trapping, and biotin-operated release. We demonstrate BOLT's capacity in synchronized secretory cargo release, microtubule-directed organelle movement and hitchhiking, and ectopically localizing nuclear transcription factor. Importantly, pulse-chase BOLT further reveals bidirectional endoplasmic reticulum-Golgi trafficking of an endogenous Golgi enzyme and distinguishes recycled from newly synthesized enzyme populations using distinct HTLs. Altogether, BOLT provides an all-in-one platform to interrogate how subcellular localization regulates protein function across HaloTag-compatible systems.",
  "abstract_shown": true,
  "affiliations": [
    "St. Jude Children's Research Hospital"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "W. C. Beyers"
    },
    {
      "name": "S. E. Plutkis"
    },
    {
      "name": "K. R. Rutcher"
    },
    {
      "name": "J. L. Sun"
    },
    {
      "name": "M. R. Haas"
    },
    {
      "name": "M. S. Hutton"
    },
    {
      "name": "H. K. Kim"
    },
    {
      "name": "A. Chavez"
    },
    {
      "name": "S. M. Pruett-Miller"
    },
    {
      "name": "J. D. Vevea"
    },
    {
      "name": "L. Lavis"
    },
    {
      "affs": [
        0
      ],
      "name": "C.-L. Chang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8zj2g1n9h4ex4.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.02.756291",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756291v1.full",
  "id": "8zj2g1n9h4ex4",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756291v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "synthetic biology"
    ],
    [
      "openalex",
      "1307|Biotin and Related Studies"
    ]
  ],
  "openalex": "W7220685038",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756291v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-02",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/8zj2g1n9h4ex4.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:09Z",
      "native_id": "10.64898/2026.10.02.756291",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.02.756291v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:synthetic biology",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Biotin-operated ligand trapping enables dynamic spatial redistribution of protein and organelle in living cells",
  "title_text": "Biotin-operated ligand trapping enables dynamic spatial redistribution of protein and organelle in living cells",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:09Z",
  "url": "https://research.draconitic.com/paper/8zj2g1n9h4ex4",
  "venue": "bioRxiv",
  "versions": []
}