{
  "abstract": "Diverse bacteria produce proteinaceous polymeric coils called R-bodies (refractile bodies), which extend into ~10 m-long needles in response to environmental triggers. It has been proposed that R-bodies deliver toxins intracellularly after such bacteria are ingested by eukaryotes, but intra-host extension of R-bodies has not been directly observed and these toxins remain unidentified. We use Pseudomonas aeruginosa PA14 and Dictyostelium discoideum to show that R-bodies extend within amoebae. We also identify a novel toxin, DeaR (deaminase associated with the R-body), which contributes to predation resistance and forms a near-megadalton dodecamer with ADP deaminase activity that alters purine metabolism. Our findings elucidate the molecular basis of R-body-mediated toxicity during PA14 infection and provide a paradigm for understanding the potential of cytotoxic effectors delivered by R-bodies (CEDRs) in interkingdom interactions.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "Devin T. King-Roberts",
      "orcid": "0009-0001-2504-4689"
    },
    {
      "name": "Kiavash Garakani",
      "orcid": "0000-0002-2896-3234"
    },
    {
      "name": "Thomas Y. Tao",
      "orcid": "0000-0002-6989-364X"
    },
    {
      "name": "Hsin-Cheng Lin",
      "orcid": "0009-0001-4135-4847"
    },
    {
      "name": "Joshua Powell"
    },
    {
      "name": "Tongxin Wu",
      "orcid": "0009-0001-3918-7429"
    },
    {
      "name": "Eugenia J. Lee"
    },
    {
      "name": "Tiffany M. Yang"
    },
    {
      "name": "Mariia Serebriakova"
    },
    {
      "name": "Alexa Price-Whelan",
      "orcid": "0000-0001-7587-7534"
    },
    {
      "name": "Virginia W. Cornish",
      "orcid": "0000-0002-5676-4718"
    },
    {
      "name": "Liang Tong",
      "orcid": "0000-0002-0563-6468"
    },
    {
      "name": "Lars E.P. Dietrich",
      "orcid": "0000-0003-2049-1137"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8zscyb3mq25xz.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.21.760852",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760852v1.full",
  "id": "8zscyb3mq25xz",
  "issns": [
    "2692-8205"
  ],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760852v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "microbiology"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1312|Bacterial biofilms and quorum sensing"
    ]
  ],
  "openalex": "W7223220652",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760852v1.full.pdf",
  "pmcid": "PMC13733733",
  "pmid": "42990220",
  "published": "2026-10-21",
  "publisher": "Cold Spring Harbor Laboratory Preprints",
  "ris": "https://research.draconitic.com/paper/8zscyb3mq25xz.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:14Z",
      "native_id": "10.64898/2026.10.21.760852",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760852v2"
    },
    {
      "first_seen": "2026-11-08T12:23:26Z",
      "native_id": "PMC13733733",
      "source": "pmc",
      "url": "https://doi.org/10.64898/2026.10.21.760852"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:microbiology",
      "subfield": "microbiology"
    }
  ],
  "title": "Pseudomonas aeruginosa R-bodies deliver an ADP deaminase toxin",
  "title_text": "Pseudomonas aeruginosa R-bodies deliver an ADP deaminase toxin",
  "type": "preprint",
  "updated_at": "2026-11-08T12:23:26Z",
  "url": "https://research.draconitic.com/paper/8zscyb3mq25xz",
  "venue": "bioRxiv",
  "versions": []
}