{
  "abstract": "Objective: To investigate the effects of the Kidney-Tonifying, Spleen-Strengthening and Blood-Activating Formula (BSJPHXF, also known as Gukang Formula) on the FGF23-Klotho bone-kidney axis and calcium-phosphorus metabolism in a rat model of postmenopausal osteoporosis.\n\nMethods: Forty SPF female Sprague-Dawley rats were randomly divided into sham-operated, ovariectomized (OVX; model), BSJPHXF and Liuwei Dihuang Wan (LWDHW; positive control) groups (n=10 per group) and treated by gavage for 4 weeks after a 12-week recovery period. Serum calcium and phosphorus were measured by automated biochemical analysis; serum FGF23, parathyroid hormone (PTH) and 1, 25-dihydroxyvitamin D ₃ [1, 25-(OH) ₂ D ₃ ] by ELISA; renal Klotho, NaPi-IIa and 1α-hydroxylase (Cyp27b1) by Western blot; bone microstructure by Micro-CT; and renal histopathology by HE staining. Serum biochemistry and ELISA were performed in nine animals per group, and Western blot, Micro-CT and histology in three animals per group; the animal was the experimental unit.\n\nResults: Compared with sham, OVX rats had lower serum calcium (1.997 ± 0.109 vs. 2.547 ± 0.189 mmol/L) and 1, 25-(OH) ₂ D ₃ (102.21 ± 2.87 vs. 201.74 ± 5.85 pg/mL) and higher serum phosphorus (2.019 ± 0.099 vs. 1.355 ± 0.027 mmol/L), FGF23 (366.82 ± 28.36 vs. 159.52 ± 20.39 pg/mL) and PTH (141.63 ± 15.29 vs. 33.91 ± 7.59 pg/mL) (all P<0.001). BSJPHXF attenuated these changes relative to OVX (P<0.001). BSJPHXF and LWDHW did not differ for calcium, FGF23 or PTH, whereas LWDHW produced a greater reduction in serum phosphorus (1.463 ± 0.062 vs. 1.587 ± 0.048 mmol/L, P = 0.0015) and a greater increase in 1, 25-(OH) ₂ D ₃ (174.66 ± 7.00 vs. 156.54 ± 13.09 pg/mL, P = 0.0002). On Micro-CT, BSJPHXF, but not LWDHW, improved trabecular bone volume fraction, separation and number versus OVX, and BSJPHXF was superior to LWDHW for all three; trabecular thickness did not differ among groups.\n\nConclusion: In OVX rats, BSJPHXF attenuated calcium-phosphorus disturbance, with accompanying changes in renal Klotho, NaPi-IIa and Cyp27b1 and in circulating FGF23, PTH and 1, 25-(OH) ₂ D ₃ , and improved trabecular microarchitecture. As the mechanistically most important endpoints rested on three animals per group and functional renal parameters were not measured, these findings are preliminary and hypothesis-generating.",
  "abstract_shown": true,
  "affiliations": [
    "The Third Clinical Medical College, Guangzhou University of Chinese Medicine",
    "The Third Affiliated Hospital of Guangzhou University of Chinese Medicine",
    "The First Clinical Medical College, Henan University of Chinese Medicine"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
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        0
      ],
      "name": "Huibing Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yinyin Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Bo Ning"
    },
    {
      "affs": [
        1
      ],
      "name": "Yanping Lin"
    },
    {
      "affs": [
        2
      ],
      "name": "Peiyu Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaoyu Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Zehua Guo"
    },
    {
      "affs": [
        1
      ],
      "name": "Genfu Zhu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/8zytqryyja1w1.bib",
  "cited_by": 0,
  "doi": "10.3389/fendo.2026.1943198",
  "fulltext_url": "https://www.frontiersin.org/articles/10.3389/fendo.2026.1943198/full",
  "id": "8zytqryyja1w1",
  "issns": [
    "1664-2392"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fendo.2026.1943198/full",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "RC648-665"
    ],
    [
      "openalex",
      "2727|Parathyroid Disorders and Treatments"
    ]
  ],
  "openalex": "W7223274910",
  "pdf_url": "https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2026.1943198/pdf",
  "pmcid": "PMC13739526",
  "pmid": "42996164",
  "published": "2026-10-23",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/8zytqryyja1w1.ris",
  "sources": [
    {
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      "url": "https://www.frontiersin.org/articles/10.3389/fendo.2026.1943198/full"
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    }
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  "subjects": [
    {
      "field": "medicine",
      "origin": "lcc:RC648-665",
      "subfield": "endocrinology-metabolism"
    }
  ],
  "title": "BSJPHXF modulates the FGF23-Klotho bone-kidney axis and calcium-phosphorus metabolism in ovariectomized rats with postmenopausal osteoporosis",
  "title_text": "BSJPHXF modulates the FGF23-Klotho bone-kidney axis and calcium-phosphorus metabolism in ovariectomized rats with postmenopausal osteoporosis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T15:20:41Z",
  "url": "https://research.draconitic.com/paper/8zytqryyja1w1",
  "venue": "Frontiers in Endocrinology",
  "versions": []
}