{
  "abstract": "The Drosophila protein male-specific lethal 1 (MSL1) is part of the five-subunit male-specific lethal (MSL) complex that binds only to the X chromosome in males. MSL1 contains a 93-amino-acid N-terminal unstructured region followed by a coiled-coil domain that forms a homodimer. The MSL1 dimer and male-specific lethal 2 (MSL2), which interacts with its coiled-coil domain, form a core that is primarily responsible for the specific recruitment of the MSL complex to X-chromosome high-affinity sites (HASs). In a yeast two-hybrid analysis, we found that the N-terminal region of MSL1, primarily amino acids 66–85 and the coiled-coil domain, interacts with many DNA-binding transcription factors. The coiled-coil domain of Drosophila virilis MSL1 (vCC) retains the ability to interact with Drosophila melanogaster MSL2 but not with zinc finger proteins. Expression of a double mutant of MSL1 (MSL1Δ20vCC), in which amino acids 66–85 (Δ20) have been deleted and the coiled-coil domain has been replaced with the vCC domain, significantly reduced dosage compensation in males due to decreased binding of the MSL complex to the X chromosome. Substitution of MSL1 with MSL1vCC in females ectopically expressing MSL2 also significantly reduced recruitment of the MSL complex to the X chromosome. Altogether, these results indicate a key role of the N-terminal region of MSL1 not only in the assembly of the MSL complex but also in its recruitment to the X chromosome in males.",
  "abstract_shown": true,
  "affiliations": [
    "Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilova Str., 119334 Moscow, Russia",
    "Center for Genome Research, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilova Str., 119334 Moscow, Russia"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Valentin Babosha",
      "orcid": "0000-0003-0402-3915"
    },
    {
      "affs": [
        0
      ],
      "name": "Varvara Ryzhkova",
      "orcid": "0000-0002-6568-7355"
    },
    {
      "affs": [
        1
      ],
      "name": "Anastasia Revel-Muroz",
      "orcid": "0000-0002-6517-1375"
    },
    {
      "affs": [
        0
      ],
      "name": "Evgeniya Tikhonova"
    },
    {
      "affs": [
        0
      ],
      "name": "Yulia Vasileva",
      "orcid": "0000-0001-5192-3073"
    },
    {
      "affs": [
        1
      ],
      "name": "Oksana Maksimenko",
      "orcid": "0000-0003-3502-0303"
    },
    {
      "affs": [
        0
      ],
      "name": "Pavel Georgiev",
      "orcid": "0000-0002-6509-3194"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/92etajck8n9fk.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27219861",
  "fulltext_url": null,
  "id": "92etajck8n9fk",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/21/9861",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [],
  "openalex": null,
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-11-03",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/92etajck8n9fk.ris",
  "sources": [
    {
      "first_seen": "2026-11-05T00:20:58Z",
      "native_id": "10.3390/ijms27219861",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/21/9861"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:1422-0067",
      "subfield": "general"
    }
  ],
  "title": "The N-Terminal Region of Drosophila MSL1 Interacts with Zinc Finger Proteins and Promotes the Recruitment of the MSL Complex to the X Chromosome in Males",
  "title_text": "The N-Terminal Region of Drosophila MSL1 Interacts with Zinc Finger Proteins and Promotes the Recruitment of the MSL Complex to the X Chromosome in Males",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T00:21:14Z",
  "url": "https://research.draconitic.com/paper/92etajck8n9fk",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}