{
  "abstract": "Despite its prominent industrial use for L-proline production, microbial fermentation by Corynebacterium glutamicum achieves a suboptimal yield of glucose conversion, far below the theoretical maximum of 0.63 g/g. This study aimed to develop an efficient engineered Escherichia coli chassis to produce L-proline from glucose by addressing the rate-limiting enzymatic step and high glucose conversion. The γ-glutamyl kinase encoding gene (proB) was screened by directed evolution, and the catalytic efficiency of the ScProB** variant toward L-glutamate increased 2.75-fold. After channeling 2-oxoglutarate flux and supplying sufficient NADPH, L-proline production reached 86.27 ± 2.58 g/L, with a 0.33 ± 0.01 g/g glucose yield in 10-L fed-batch fermentation. Further increasing the flux through central carbon metabolism by implementing a non-PTS glucose uptake system, amplifying the anaplerotic reaction, and redirecting the flux away from the glyoxylate cycle, the resulting strain BP23 achieved 119.89 ± 5.66 g/L L-proline, with a productivity of 1.93 ± 0.03 g/L/h. The glucose conversion yield improved to 0.49 ± 0.01 g/g after 62 h of fed-batch fermentation, indicating that the E. coli strain is a promising workhorse for producing L-proline with high glucose conversion.",
  "abstract_shown": true,
  "affiliations": [
    "National and Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fujian, People's Republic of China.",
    "National and Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fujian, People's Republic of China. npkace@fjnu.edu.cn.",
    "State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.",
    "National and Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fujian, People's Republic of China. hjz@fjnu.edu.cn.",
    "National and Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology, Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fujian, People's Republic of China. kechr@fjnu.edu.cn."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Shumei Cui"
    },
    {
      "affs": [
        0
      ],
      "name": "Xiaolu Hu"
    },
    {
      "affs": [
        1
      ],
      "name": "Xinwei Yang",
      "orcid": "0000-0003-0776-8269"
    },
    {
      "affs": [
        0
      ],
      "name": "Yongtao Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Tianqi Bao"
    },
    {
      "affs": [
        0
      ],
      "name": "Bingyan Wang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yong Tao"
    },
    {
      "affs": [
        3
      ],
      "name": "Jianzhong Huang"
    },
    {
      "affs": [
        4
      ],
      "name": "Chongrong Ke"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/93gsn2n1qzyf2.bib",
  "cited_by": 0,
  "doi": "10.1186/s12934-026-03075-x",
  "fulltext_url": "https://europepmc.org/articles/PMC13718036",
  "id": "93gsn2n1qzyf2",
  "issns": [
    "1475-2859"
  ],
  "landing_url": "https://europepmc.org/article/MED/42958551",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Research"
    ],
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W7171694509",
  "pdf_url": "https://europepmc.org/articles/PMC13718036?pdf=render",
  "pmcid": "PMC13718036",
  "pmid": "42958551",
  "published": "2026-07-29",
  "publisher": "BMC",
  "ris": "https://research.draconitic.com/paper/93gsn2n1qzyf2.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T00:20:48Z",
      "native_id": "MED:42958551",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42958551"
    },
    {
      "first_seen": "2026-11-02T12:22:11Z",
      "native_id": "PMC13718036",
      "source": "pmc",
      "url": "https://doi.org/10.1186/s12934-026-03075-x"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Efficient Escherichia coli platform for L-proline biosynthesis via systematic metabolic engineering.",
  "title_text": "Efficient Escherichia coli platform for L-proline biosynthesis via systematic metabolic engineering.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:11Z",
  "url": "https://research.draconitic.com/paper/93gsn2n1qzyf2",
  "venue": "Microbial cell factories",
  "versions": []
}