{
  "abstract": "Sarcopenia, characterized by the progressive loss of skeletal muscle mass and function, is a major hallmark of aging. Post-translational modifications (PTMs) play essential roles in regulating protein activity and cellular homeostasis; however, how multiple PTMs are remodeled during skeletal muscle aging remains incompletely characterized. Here, we performed comprehensive multi-layered proteomic profiling of skeletal muscle from young (3-month-old) and aged (24-month-old) mice, systematically quantifying the global proteome together with five major PTMs: acetylation, phosphorylation, N-glycosylation, O-glycosylation, and ubiquitination. In total, we identified 5337 proteins and mapped thousands of PTM sites, generating an integrated atlas of age-associated proteomic and PTM remodeling in skeletal muscle. Pathway enrichment analyses revealed distinct modification-specific patterns: acetylation and phosphorylation were predominantly associated with metabolic and mitochondrial-related pathways; N-glycosylation was enriched in immune- and secretory pathway-related processes; O-glycosylation was associated with muscle contraction-related pathways; and ubiquitination was preferentially linked to cytoskeletal organization in muscle cells. Correlation analyses further uncovered diverse association patterns among different PTMs across protein- and modification-level datasets. Phosphorylation and ubiquitination exhibited consistent positive associations, whereas acetylation and ubiquitination showed both inverse and concordant co-variation patterns across subsets of proteins. Phosphorylation and O-glycosylation displayed heterogeneous association patterns across different proteins, and acetylation and phosphorylation demonstrated positive correlations with distinct age-associated directional changes across protein subsets. Together, these results provide a comprehensive, multi-dimensional view of age-associated remodeling of the skeletal muscle proteome and multiple PTM layers, offering a valuable resource for understanding molecular alterations accompanying muscle aging and sarcopenia.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Sports Medicine, The Beijing Jishuitan Hospital Guizhou Hospital, Guiyang, Guizhou 550014, China",
    "Metabolic Control and Aging, Human Aging Research Institute and School of Life Science, Nanchang University, and Jiangxi Key Laboratory of Human Aging, Nanchang, Jiangxi 330031, China",
    "Department of Urology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550001, China",
    "HeartSeek Biotechnology, Kunming, Yunnan 650000, China",
    "Institutional Center for Shared Technologies and Facilities of the Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650201, China",
    "Aging and Vascular Diseases, Human Aging Research Institute and School of Life Science, Nanchang University, and Jiangxi Key Laboratory of Aging and Diseases, Nanchang, Jiangxi 330031, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jian Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Xin-Qiang Lan"
    },
    {
      "affs": [
        2
      ],
      "name": "Cai-Fen Guo"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiang Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Chun-Ping Huang"
    },
    {
      "affs": [
        3
      ],
      "name": "Dong-Qin Zhang"
    },
    {
      "affs": [
        4
      ],
      "name": "Lin Zeng"
    },
    {
      "affs": [
        5
      ],
      "name": "Xiao-Li Tian"
    },
    {
      "affs": [
        1
      ],
      "name": "Yang Xiang"
    },
    {
      "affs": [
        1
      ],
      "name": "Qi-Quan Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/93n4xfegpska4.bib",
  "cited_by": 0,
  "doi": "10.24272/j.issn.2095-8137.2025.460",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13720437/",
  "id": "93n4xfegpska4",
  "issns": [
    "2095-8137"
  ],
  "landing_url": "https://doi.org/10.24272/j.issn.2095-8137.2025.460",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1312|Muscle Physiology and Disorders"
    ]
  ],
  "openalex": "W7133196080",
  "pdf_url": "https://www.zoores.ac.cn/en/article/pdf/preview/10.24272/j.issn.2095-8137.2025.460.pdf",
  "pmcid": "PMC13720437",
  "pmid": "42706783",
  "published": "2026-09-18",
  "publisher": "Editorial Office of Zoological Research, Kunming Institute of Zoology, The Chinese Academy of Sciences",
  "ris": "https://research.draconitic.com/paper/93n4xfegpska4.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:21:19Z",
      "native_id": "PMC13720437",
      "source": "pmc",
      "url": "https://doi.org/10.24272/j.issn.2095-8137.2025.460"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Muscle Physiology and Disorders",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Comprehensive multi-post-translational modifications profiling reveals age-associated remodeling in skeletal muscle",
  "title_text": "Comprehensive multi-post-translational modifications profiling reveals age-associated remodeling in skeletal muscle",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:21:19Z",
  "url": "https://research.draconitic.com/paper/93n4xfegpska4",
  "venue": "Zoological Research",
  "versions": []
}