{
  "abstract": "Tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine (DA) synthesis, is conserved across most animal phyla. DA regulates motor activity, sensory processing motivation, neural homeostasis, and protection against oxidative stress. TH and DA have been described in vertebrates and in some invertebrates, including arthropods. This study investigated the anatomical location of TH and DA and characterized the biochemical and molecular structure of TH in the brain of the mangrove crab Ucides cordatus. This species is a useful environmental model for neurodegeneration studies, due to its occurrence in metal-contaminated mangrove ecosystems, and as prey of other species, including humans. Using immunohistochemistry, immunoblotting, and structural modeling, we mapped TH presence and distribution in the crustacean brain and analyzed its molecular and evolutionary characteristics. Our results revealed a TH fragment structurally similar to fragments found in 21 other species and in a complex dopaminergic network in U. cordatus, characterized by diverse neuronal morphologies, widespread projections, and sensitivity to dopaminergic neurotoxins. These findings may support further studies on catecholaminergic neurobiology.",
  "abstract_shown": true,
  "affiliations": [
    "Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.",
    "Programa de Pós-Graduação em Ciências Biológicas (Biofísica), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-902, Brazil.",
    "Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.",
    "Centre de Recherche du CHU de Québec, Axe Neurosciences, 2705, Boulevard Laurier, Québec, QC, G1V 4G2, Canada.",
    "Center for Integration of Science and Industry, Bentley University, Waltham, MA, 02452, USA.",
    "Faculdade de Fisioterapia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 22290-140, Brazil.",
    "Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil. sallodi@biof.ufrj.br.",
    "Programa de Pós-Graduação em Ciências Biológicas (Biofísica), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-902, Brazil. sallodi@biof.ufrj.br."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Carlos Augusto Borges de Andrade Gomes"
    },
    {
      "affs": [
        2,
        3
      ],
      "name": "Thyago R Cardim-Pires",
      "orcid": "0000-0002-9498-8480"
    },
    {
      "affs": [
        0
      ],
      "name": "Rafaela Rocha Dos Santos"
    },
    {
      "affs": [
        0
      ],
      "name": "Maria Clara da Costa Simas"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Andressa de Abreu Mello"
    },
    {
      "affs": [
        0
      ],
      "name": "Louise Caroline Vitorino"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Paula Grazielle Chaves da Silva"
    },
    {
      "affs": [
        2
      ],
      "name": "Debora Foguel"
    },
    {
      "affs": [
        5
      ],
      "name": "Clynton Lourenço Correa"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Rosane Silva"
    },
    {
      "affs": [
        6,
        7
      ],
      "name": "Silvana Allodi",
      "orcid": "0000-0002-3053-3866"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/93tarpe17sqqr.bib",
  "cited_by": 0,
  "doi": "10.1007/s12035-026-06269-x",
  "fulltext_url": "https://europepmc.org/articles/PMC13683526",
  "id": "93tarpe17sqqr",
  "issns": [
    "0893-7648",
    "1559-1182"
  ],
  "landing_url": "https://europepmc.org/article/MED/42900443",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2804|Neurobiology and Insect Physiology Research"
    ]
  ],
  "openalex": "W7223029198",
  "pdf_url": "https://europepmc.org/articles/PMC13683526?pdf=render",
  "pmcid": "PMC13683526",
  "pmid": "42900443",
  "published": "2026-10-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/93tarpe17sqqr.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:32Z",
      "native_id": "MED:42900443",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42900443"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2804|Neurobiology and Insect Physiology Research",
      "subfield": "neuroscience"
    }
  ],
  "title": "Unraveling Tyrosine Hydroxylase in the Brain of a Crustacean: An Integrative Investigation Using Different Approaches.",
  "title_text": "Unraveling Tyrosine Hydroxylase in the Brain of a Crustacean: An Integrative Investigation Using Different Approaches.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:32Z",
  "url": "https://research.draconitic.com/paper/93tarpe17sqqr",
  "venue": "Molecular neurobiology",
  "versions": []
}