{
  "abstract": "Background/Objectives: Botanical metabolites can modulate cellular stress responses, lipid homeostasis, and epithelial organization. Arctium lappa L. (Burdock) root contains bioactive phytochemicals and inulin-type fructans, but its molecular effects in vitro remain incompletely characterized. Methods: HaCaT keratinocytes were exposed to a standardized aqueous Burdock root extract (1 mg/mL) for 4.5 h and analyzed by RNA sequencing. The Burdock-versus-control response was evaluated using gene-level differential-expression analysis, ReactomeGSA, and focused GO and KEGG ORA. Higher-amplitude responses were subsequently compared with those induced under identical conditions by Rosehip and Schisandra extracts. Results: Burdock treatment identified 80 DEGs meeting FDR ≤ 0.05 and |log₂ FC| ≥ 1. The response was characterized by induction of metallothioneins, zinc transporters, NRF2- and AhR-associated genes. Increased expression of selected sterol-transport and intracellular-handling genes was accompanied by coordinated downregulation of cholesterol- and lanosterol-biosynthesis pathways. Reciprocal regulation of SPHK1, SGPP1, and SGPL1 indicated altered transcriptional control of S1P production and turnover. Junctional, cytoskeletal, TGF-β-associated, and epithelial cell-state genes were also regulated. Comparative analysis identified 29 higher-amplitude DEGs meeting the predefined Burdock threshold criteria but not the corresponding comparator thresholds, including an 11-gene subgroup not significantly regulated by either comparator treatment. Conclusions: Together, these findings define an early transcriptional response integrating metal homeostasis, cytoprotective signaling, sterol and sphingolipid metabolism, and epithelial remodeling, providing a mechanistic framework for future investigation of Burdock rootextract nutritional and dermatological research.",
  "abstract_shown": true,
  "affiliations": [
    "ABC-Bioresearch, 37126 Verona, Italy.",
    "Martin Bauer Nutraceuticals Srl, 10121 Torino, Italy.",
    "Finzelberg GmbH &CoKG, 56266 Andernach, Germany.",
    "Department of Neurosciences, Biomedicine and Movement Sciences, School of Medicine, University of Verona, 37134 Verona, Italy.",
    "Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Cecilia Bender",
      "orcid": "0000-0001-9510-9823"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Cynthia Gisela Suarez",
      "orcid": "0000-0002-9568-6556"
    },
    {
      "affs": [
        0
      ],
      "name": "Alejandra Mariel Habarta"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Andressa Blainski",
      "orcid": "0000-0002-4935-5270"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Alessandra Ruggiero"
    },
    {
      "affs": [
        4
      ],
      "name": "Mariano Stornaiuolo",
      "orcid": "0000-0003-2200-5083"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/953v80twprkxg.bib",
  "cited_by": 0,
  "doi": "10.3390/metabo16100707",
  "fulltext_url": "https://europepmc.org/articles/PMC13707041",
  "id": "953v80twprkxg",
  "issns": [
    "2218-1989"
  ],
  "landing_url": "https://europepmc.org/article/MED/42941183",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2708|Skin Protection and Aging"
    ]
  ],
  "openalex": "W7214161885",
  "pdf_url": "https://europepmc.org/articles/PMC13707041?pdf=render",
  "pmcid": "PMC13707041",
  "pmid": "42941183",
  "published": "2026-09-24",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/953v80twprkxg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42941183",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42941183"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-1989",
      "subfield": "proteomics-metabolomics"
    }
  ],
  "title": "Burdock (Arctium lappa L.) Root Extract Induces Transcriptional Signatures Associated with Metal Homeostasis and Lipid Metabolism in Human Keratinocytes.",
  "title_text": "Burdock (Arctium lappa L.) Root Extract Induces Transcriptional Signatures Associated with Metal Homeostasis and Lipid Metabolism in Human Keratinocytes.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/953v80twprkxg",
  "venue": "Metabolites",
  "versions": []
}