{
  "abstract": "Critical-size segmental bone defects remain a major challenge in orthopedic surgery, often requiring complex reconstruction strategies associated with significant morbidity. This study evaluated the regenerative potential of a self-crosslinking bone substitute (SCBS) composed of biphasic calcium phosphate (BCP) granules suspended in a silanized hydroxypropyl methylcellulose (Si-HPMC) hydrogel, with or without recombinant human bone morphogenetic protein-2 (rhBMP-2), in a canine load-bearing defect model. Bilateral 2-cm segmental defects were created in the ulnae of five adult beagle dogs. Defects were filled with SCBS alone or SCBS loaded with rhBMP-2. Bone regeneration and biomaterial remodeling were assessed after 20 weeks using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), histomorphometry, elemental analysis, and histology. SCBS loaded with rhBMP-2 resulted in complete defect bridging, with 35% newly formed bone and only 5% residual BCP granules. In contrast, SCBS alone induced limited bone formation (10%), primarily at host interfaces, with substantial persistence of BCP (33%). Newly formed bone in the rhBMP-2 group exhibited a dense lamellar structure with Haversian organization and direct contact with residual biomaterial. Elemental analysis revealed a lower Ca/P ratio compared with control, suggesting ongoing remodeling. These findings demonstrate that controlled delivery of rhBMP-2 from a self-crosslinking CaP/hydrogel composite enhances both bone formation and biomaterial resorption, supporting a coupled regeneration process. This approach represents a promising strategy for the treatment of segmental bone defects and non-unions in orthopedic applications.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Amadou Touré"
    },
    {
      "name": "Ombeline Aroux"
    },
    {
      "name": "Joelle Veziers"
    },
    {
      "name": "Sophie Sourice"
    },
    {
      "name": "Kevin Minier"
    },
    {
      "name": "Borhane Fellah"
    },
    {
      "name": "Valérie Geoffroy"
    },
    {
      "name": "Bernard Giumelli"
    },
    {
      "name": "Olivier Gauthier"
    },
    {
      "name": "Pierre Weiss"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/97vgbrqyrszxp.bib",
  "cited_by": 0,
  "doi": "10.3390/bioengineering13080888",
  "fulltext_url": null,
  "id": "97vgbrqyrszxp",
  "issns": [
    "2306-5354"
  ],
  "landing_url": "https://hal.science/hal-05740638v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "2204|Bone Tissue Engineering Materials"
    ]
  ],
  "openalex": "W7172334066",
  "pdf_url": "https://hal.science/hal-05740638/document",
  "pmcid": null,
  "pmid": "42649778",
  "published": "2026-07-31",
  "publisher": "MDPI",
  "ris": "https://research.draconitic.com/paper/97vgbrqyrszxp.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:29Z",
      "native_id": "hal-05740638",
      "source": "hal",
      "url": "https://hal.science/hal-05740638v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    }
  ],
  "title": "BMP-2-Loaded Self-Crosslinking CaP/Hydrogel Composite Enables Complete Regeneration of Critical-Sized Segmental Bone Defects",
  "title_text": "BMP-2-Loaded Self-Crosslinking CaP/Hydrogel Composite Enables Complete Regeneration of Critical-Sized Segmental Bone Defects",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:29Z",
  "url": "https://research.draconitic.com/paper/97vgbrqyrszxp",
  "venue": "Bioengineering",
  "versions": []
}