{
  "abstract": "We present a portable, low-cost, modular spectrophotometric platform designed for wavelength-selective optical measurements using interchangeable visible LEDs, an AS7343 multichannel spectral sensor, and Arduino-based data acquisition. The system was developed to provide a compact alternative for optical absorbance and fluorescence measurements while maintaining performance comparable to conventional laboratory instrumentation. The device was evaluated using aqueous solutions of copper (II) sulfate (CuSO₄) and potassium permanganate (KMnO₄), and results were benchmarked against a standard UV-Vis spectrophotometer. The system demonstrated concentration-dependent absorbance responses consistent with the Beer-Lambert law, with strong calibration linearity (R ² = 0.99 for CuSO₄ and R ² = 0.97 for KMnO₄). In addition, the platform was adapted for fluorescence measurements of Rhodamine B via a simple reconfiguration to a 90° excitation-emission geometry. The fluorescence response showed linear behavior over the 0-4-μM range, yielding an R ² value of 0.97. The results indicate that the proposed system provides reliable optical measurements for both absorbance and fluorescence modalities, offering a flexible and cost-effective alternative for analytical spectrophotometry applications in resource-limited laboratory settings.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Chemistry Florida Agricultural and Mechanical University Tallahassee 32307 Florida USA famu.edu.",
    "Food Science Program College of Agriculture and Food Sciences Florida Agricultural and Mechanical University Tallahassee 32307 Florida USA famu.edu.",
    "School of the Environment Florida Agricultural and Mechanical University Tallahassee 32307 Florida USA famu.edu."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sanam Pudasaini",
      "orcid": "0000-0001-9782-3369"
    },
    {
      "affs": [
        1
      ],
      "name": "Prakash Dangal",
      "orcid": "0009-0009-3867-7379"
    },
    {
      "affs": [
        2
      ],
      "name": "Rajesh Singh Rathore"
    },
    {
      "affs": [
        0
      ],
      "name": "Beni B Dangi",
      "orcid": "0000-0002-1866-8725"
    },
    {
      "affs": [
        1
      ],
      "name": "Vijay Singh Chhetri",
      "orcid": "0000-0002-9296-6798"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/99crf1wy0xp8f.bib",
  "cited_by": 0,
  "doi": "10.1155/jamc/9371485",
  "fulltext_url": "https://europepmc.org/articles/PMC13717223",
  "id": "99crf1wy0xp8f",
  "issns": [
    "2090-8865",
    "2090-8873"
  ],
  "landing_url": "https://europepmc.org/article/MED/42959564",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "heading",
      "Research Article"
    ],
    [
      "openalex",
      "1502|Analytical Chemistry and Sensors"
    ]
  ],
  "openalex": "W7224446709",
  "pdf_url": "https://europepmc.org/articles/PMC13717223?pdf=render",
  "pmcid": "PMC13717223",
  "pmid": "42959564",
  "published": "2026-10-28",
  "publisher": "Wiley",
  "ris": "https://research.draconitic.com/paper/99crf1wy0xp8f.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T00:20:50Z",
      "native_id": "MED:42959564",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42959564"
    },
    {
      "first_seen": "2026-11-02T12:21:34Z",
      "native_id": "PMC13717223",
      "source": "pmc",
      "url": "https://doi.org/10.1155/jamc/9371485"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1502|Analytical Chemistry and Sensors",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "A Portable, Low-Cost Spectrophotometer for Absorbance and Selected-Wavelength Fluorescence Measurements.",
  "title_text": "A Portable, Low-Cost Spectrophotometer for Absorbance and Selected-Wavelength Fluorescence Measurements.",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:21:34Z",
  "url": "https://research.draconitic.com/paper/99crf1wy0xp8f",
  "venue": "Journal of analytical methods in chemistry",
  "versions": []
}