{
  "abstract": "Hepatitis E virus genotype 3 (HEV-3) is a zoonotic pathogen whose burden in Latin America remains underestimated. We conducted a cross-sectional One Health surveillance study (2019-2024) in Quindío, Colombia, testing 42 pig fecal, 8 farm runoff, 4 tributary water samples, and 5 clinical serum samples from patients with hepatitis of undetermined etiology by reverse transcription quantitative PCR (RT-qPCR). Positive and selected clinical/environmental samples underwent Oxford Nanopore MinION sequencing targeting the open reading frame 2 and 3 (ORF2/ORF3) junction, k-mer fingerprinting (k = 25), consensus generation, phylogenetic analysis, and Sanger confirmation. HEV RNA was detected in 8/42 pig fecal samples (19.0%), 4/8 runoff samples (50.0%), 1/4 tributary samples (25.0%), and 4/5 serum samples (80.0%). K-mer analysis identified strong HEV signals in two clinical samples (44.5% and 39.5% of reads) and wastewater (97.2%). Phylogenetic analysis classified Colombian isolates within HEV-3 (bootstrap 83%), while Sanger sequencing confirmed HEV-specific motifs. Two sequences were deposited in GenBank (PZ262436, PZ262437), representing the first Colombian HEV-3 genomic records. These findings demonstrate HEV-3 circulation across porcine, environmental, and human compartments and highlight substantial genomic divergence, supporting locally calibrated molecular approaches and strengthened One Health genomic surveillance in Colombia and Latin America.",
  "abstract_shown": true,
  "affiliations": [
    "GEPAMOL/GYMOL Research Group, Faculty of Health Sciences, Universidad del Quindío, Armenia 630004, Colombia."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Maria Mercedes González",
      "orcid": "0000-0001-5630-5557"
    },
    {
      "affs": [
        0
      ],
      "name": "Omar David Moreno",
      "orcid": "0000-0001-5069-6008"
    },
    {
      "affs": [
        0
      ],
      "name": "Leonardo Padilla-Sanabria",
      "orcid": "0000-0001-5811-2791"
    },
    {
      "affs": [
        0
      ],
      "name": "Jhon Carlos Castaño-Osorio",
      "orcid": "0000-0002-7639-3053"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9a5721f53e99q.bib",
  "cited_by": 0,
  "doi": "10.3390/tropicalmed11100290",
  "fulltext_url": "https://europepmc.org/articles/PMC13709530",
  "id": "9a5721f53e99q",
  "issns": [
    "2414-6366"
  ],
  "landing_url": "https://europepmc.org/article/MED/42931475",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2721|Hepatitis Viruses Studies and Epidemiology"
    ]
  ],
  "openalex": "W7221669881",
  "pdf_url": "https://europepmc.org/articles/PMC13709530?pdf=render",
  "pmcid": "PMC13709530",
  "pmid": "42931475",
  "published": "2026-10-13",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9a5721f53e99q.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:01Z",
      "native_id": "MED:42931475",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42931475"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2721|Hepatitis Viruses Studies and Epidemiology",
      "subfield": "general"
    }
  ],
  "title": "Hepatitis E Virus Genotype 3 in Quindío, Colombia: One Health Surveillance and First Genomic Characterization Reveal Cryptic Regional Divergence.",
  "title_text": "Hepatitis E Virus Genotype 3 in Quindío, Colombia: One Health Surveillance and First Genomic Characterization Reveal Cryptic Regional Divergence.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:01Z",
  "url": "https://research.draconitic.com/paper/9a5721f53e99q",
  "venue": "Tropical medicine and infectious disease",
  "versions": []
}