{
  "abstract": "tRNA fragments (tRFs) are natural, small non-coding RNAs produced by endogenous ribonucleolytic cleavage of tRNAs. tRFs play a role in many biological pathways, including regulation of cell proliferation. Specific tRNA modifications affect tRF biogenesis. Queuosine (Q) modification occurs at the wobble anticodon nucleotide of 4 mammalian tRNAs and protects these tRNAs from fragmentation. Here, we investigated cell-type and tRNA Q-modification dependencies on mammalian cell proliferation. We found that Q-modified tRNA can either increase, decrease, or make no difference in proliferation. Proliferation decreased in MCF7, a breast cancer cell line, when tRNAs were Q-modified and dependent on the modification enzyme complex, QTRT1/2. MCF7 proliferation increases upon the transfection of Q-modification-dependent tRFHis or decreases upon knockdown of endogenous tRFHis. Consistent with Q-dependent proliferation reduction, polysome profiling showed decreased translation efficiency of ribosomal proteins. Finally, protein pull-down of MCF7 lysates using tRFHis identified Musashi RNA-binding protein 2 (Msi2) as a tRFHis-binding protein. Msi2 knockdown reduced the Q-modification-dependent or tRFHis dependent cell proliferation effect. Our results illustrate a highly cell-type-dependent proliferation effect of tRNA Q-modification, suggesting multi-faceted mechanisms in studying this ubiquitous tRNA modification.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry and Molecular Biology, University of Chicago , Chicago, IL 60637 ,",
    "Department of Molecular Genetics and Cell Biology, University of Chicago , Chicago, IL 60637 ,",
    "Committee on Microbiology, University of Chicago , Chicago, IL 60637 ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Wen Zhang",
      "orcid": "0000-0001-8577-2613"
    },
    {
      "affs": [
        1
      ],
      "name": "Brandon J Chew"
    },
    {
      "affs": [
        0
      ],
      "name": "Zaneta Matuszek"
    },
    {
      "affs": [
        0
      ],
      "name": "Sihao Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Luke R Frietze"
    },
    {
      "affs": [
        0
      ],
      "name": "Hoang Anh V Tran"
    },
    {
      "affs": [
        0
      ],
      "name": "Christopher D Katanski"
    },
    {
      "affs": [
        2
      ],
      "name": "Marcus Foo"
    },
    {
      "affs": [
        0
      ],
      "name": "Qing Dai"
    },
    {
      "affs": [
        0
      ],
      "name": "Tao Pan",
      "orcid": "0000-0003-1096-8405"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9a7rm6mk5srdr.bib",
  "cited_by": 0,
  "doi": "10.1093/nar/gkag1101",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13722870/?tool=EBI",
  "id": "9a7rm6mk5srdr",
  "issns": [
    "0305-1048",
    "1362-4962"
  ],
  "landing_url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1101/9005797",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "AcademicSubjects/SCI00010"
    ],
    [
      "heading",
      "Chemical Biology and Nucleic Acid Chemistry"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://academic.oup.com/nar/article-pdf/54/20/gkag1101/72640298/gkag1101.pdf",
  "pmcid": "PMC13722870",
  "pmid": "42965288",
  "published": "2026-10-31",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/9a7rm6mk5srdr.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:18Z",
      "native_id": "10.1093/nar/gkag1101",
      "source": "crossref",
      "url": "https://academic.oup.com/nar/article/doi/10.1093/nar/gkag1101/9005797"
    },
    {
      "first_seen": "2026-11-02T03:21:51Z",
      "native_id": "PMC:PMC13722870",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13722870"
    },
    {
      "first_seen": "2026-11-04T12:21:36Z",
      "native_id": "PMC13722870",
      "source": "pmc",
      "url": "https://doi.org/10.1093/nar/gkag1101"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:0305-1048",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Queuosine-modification dependent histidyl tRNA fragment promotes cell-type dependent proliferation",
  "title_text": "Queuosine-modification dependent histidyl tRNA fragment promotes cell-type dependent proliferation",
  "type": "peer-reviewed",
  "updated_at": "2026-11-04T12:21:36Z",
  "url": "https://research.draconitic.com/paper/9a7rm6mk5srdr",
  "venue": "Nucleic Acids Research",
  "versions": []
}