{
  "abstract": "Cystic fibrosis (CF) airway disease is characterized by exaggerated inflammatory responses and progressive respiratory failure. Although interleukin-8 (IL-8) is a central mediator of neutrophilic inflammation in the CF airway, the upstream epithelial pathways that regulate cytokine production remain incompletely understood. Interleukin-17C (IL-17C) is an epithelial-derived IL-17 family cytokine that can promote inflammatory signaling in an autocrine/paracrine manner, but its regulation in CF-derived airway epithelial cells remains poorly defined. Here, we examined Toll-like receptor (TLR) ligand-induced IL-17C regulation in primary human airway epithelial cells derived from bronchial and small airway compartments, including normal human bronchial epithelial cells, CF-derived human bronchial epithelial cells, small airway epithelial cells (SAEC), and CF-derived SAEC (D-SAEC-CF). Among the ligands tested, polyinosinic-polycytidylic acid [poly(I:C)], a synthetic TLR3 ligand, consistently revealed differences in IL-17C and IL-8 induction between non-CF and CF-derived cells in both epithelial cell systems. Time-course analyses showed that poly(I:C)-induced IL-17C expression increased gradually and exhibited relatively delayed kinetics compared with the rapid interferon-β response, resembling IL-8 induction. Pharmacological inhibition indicated that c-Jun N-terminal kinase, p38 mitogen-activated protein kinase, and nuclear factor-κB, but not extracellular signal-regulated kinase, were involved in poly(I:C)-induced IL-17C expression. Basal TLR3 and IL-17RE mRNA levels were elevated most clearly in D-SAEC-CF cells; however, IL-17RE knockdown did not significantly reduce poly(I:C)-induced IL-8 production in bronchial epithelial cells. Together, these findings suggest that poly(I:C)-induced IL-17C production may represent a TLR3-dependent epithelial inflammatory response in CF-derived primary airway epithelial cells.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Keiko Ueno-Shuto"
    },
    {
      "name": "Megumi Hayashi"
    },
    {
      "name": "Yukihiro Tasaki"
    },
    {
      "name": "Shunsuke Kamei"
    },
    {
      "name": "Kouhei Onuki"
    },
    {
      "name": "Mary Ann Suico"
    },
    {
      "name": "Hirofumi Kai"
    },
    {
      "name": "Tsuyoshi Shuto"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/9ade9gqe9dp3y.bib",
  "cited_by": 0,
  "doi": "10.1248/bpb.b26-00266",
  "fulltext_url": "https://www.jstage.jst.go.jp/article/bpb/49/7/49_b26-00266/_article",
  "id": "9ade9gqe9dp3y",
  "issns": [
    "1347-5215",
    "0918-6158"
  ],
  "landing_url": "https://www.jstage.jst.go.jp/article/bpb/49/7/49_b26-00266/_article",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "jstage-journal",
      "medicine/pharmacology-toxicology"
    ],
    [
      "openalex",
      "2740|Cystic Fibrosis Research Advances"
    ]
  ],
  "openalex": "W7171430395",
  "pdf_url": "https://www.jstage.jst.go.jp/article/bpb/49/7/49_b26-00266/_pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-28",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/9ade9gqe9dp3y.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:32Z",
      "native_id": "10.1248/bpb.b26-00266",
      "source": "jstage",
      "url": "https://www.jstage.jst.go.jp/article/bpb/49/7/49_b26-00266/_article"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "jstage-journal:medicine/pharmacology-toxicology",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Increased Interleukin-17C Production by the Toll-Like Receptor 3 Ligand Poly(I:C) in Primary Cystic Fibrosis Airway Epithelial Cells",
  "title_text": "Increased Interleukin-17C Production by the Toll-Like Receptor 3 Ligand Poly(I:C) in Primary Cystic Fibrosis Airway Epithelial Cells",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:32Z",
  "url": "https://research.draconitic.com/paper/9ade9gqe9dp3y",
  "venue": "Biological and Pharmaceutical Bulletin",
  "versions": []
}